Synthesis of oxidized sodium alginate and its electrospun bio-hybrids with zinc oxide nanoparticles to promote wound healing

Wei Wang; MingYue Liu; Muhammad Shafiq; HaiYan Li; Rashida Hashim; Mohamed El-Newehy; Hany El-Hamshary; Yosry Morsi; Xiumei Mo

doi:10.1016/j.ijbiomac.2023.123480

Synthesis of oxidized sodium alginate and its electrospun bio-hybrids with zinc oxide nanoparticles to promote wound healing

Int J Biol Macromol. 2023 Mar 31:232:123480. doi: 10.1016/j.ijbiomac.2023.123480. Epub 2023 Jan 28.

Authors

Wei Wang¹, MingYue Liu¹, Muhammad Shafiq², HaiYan Li¹, Rashida Hashim³, Mohamed El-Newehy⁴, Hany El-Hamshary⁴, Yosry Morsi⁵, Xiumei Mo⁶

Affiliations

¹ State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Biological Science and Medical Engineering, Donghua University, Shanghai, 201620, China.
² State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Biological Science and Medical Engineering, Donghua University, Shanghai, 201620, China; Department of Chemical Engineering, Faculty of Chemical Engineering, Graduate School, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, Japan. Electronic address: shafiqdr786@yahoo.com.
³ School of Physical Sciences, University of Punjab (PU), Lahore 54000, Pakistan.
⁴ Department of Chemistry, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
⁵ Faculty of Engineering and Industrial Sciences, Swinburne University of Technology, Boroondara, VIC 3122, Australia.
⁶ State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Biological Science and Medical Engineering, Donghua University, Shanghai, 201620, China. Electronic address: xmm@dhu.edu.cn.

PMID: 36720331
DOI: 10.1016/j.ijbiomac.2023.123480

Abstract

Electrospun fibers provide a promising platform for wound healing; however, they lack requisite characteristics for wound repair, including antibacterial and anti-inflammatory properties and angiogenic ability. Sodium alginate (SA) is being used for different types of applications. However, the poor spinnability of SA restricts its applications. The objectives of this study were three-fold: a) to synthesize oxidized sodium alginate (OSA) to improve its spinnability, b) to fabricate composite fibrous membranes by blending OSA along with zinc oxide nanoparticles (ZnO-NPs), and c) to decipher antibacterial and anti-inflammatory properties as well as biocompatibility of membranes in vitro and in vivo. OSA displaying different oxidation degrees (Dox (%)) was synthesized by varying the molar ratio of sodium periodate to SA. OSA (Dox, ∼48 %) afforded smooth and uniform fibers; 0.5 wt% of adipic dihydrazide (ADH) evolved into structurally stable and water-insoluble membranes. Composite fibrous membranes containing 2 wt% of ZnO-NPs displayed good biocompatibility and bactericidal effect against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) in vitro. In addition, composite membranes showed remarkable epithelialization, neovascularization, and anti-inflammatory response than that of the membranes devoid of ZnO-NPs. Conclusively, these composite fibrous membranes may have broad implications for wound healing applications.

Keywords: Electrospinning; Functionalization; Sodium alginate; Tissue engineering; Wound dressing; ZnO-NPs.

MeSH terms

Alginates
Anti-Bacterial Agents / pharmacology
Anti-Bacterial Agents / therapeutic use
Escherichia coli
Nanoparticles*
Staphylococcus aureus
Wound Healing
Zinc Oxide* / pharmacology

Substances

Alginates
Anti-Bacterial Agents
Zinc Oxide