Prevalence and antimicrobial resistance of Salmonella and ESBL E. coli isolated from dairy cattle in Henan Province, China

Jie Wang; Xiaojie Zhu; Yuxi Zhao; Huan Liu; Zhen Zhang; Lei Yan; Yingyu Chen; Ian D Robertson; Aizhen Guo; Joshua W Aleri

doi:10.1016/j.prevetmed.2023.105856

Prevalence and antimicrobial resistance of Salmonella and ESBL E. coli isolated from dairy cattle in Henan Province, China

Prev Vet Med. 2023 Apr:213:105856. doi: 10.1016/j.prevetmed.2023.105856. Epub 2023 Jan 23.

Authors

Jie Wang¹, Xiaojie Zhu², Yuxi Zhao³, Huan Liu⁴, Zhen Zhang⁵, Lei Yan⁶, Yingyu Chen⁷, Ian D Robertson⁸, Aizhen Guo⁹, Joshua W Aleri¹⁰

Affiliations

¹ The State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China; School of Veterinary Medicine, College of Science, Health, Engineering and Education, Murdoch University, 90 South Street, Murdoch, 6150 Western Australia, Australia; Hubei International Scientific and Technological Cooperation Base of Veterinary Epidemiology, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: Jie.Wang@murdoch.edu.au.
² The State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China; School of Veterinary Medicine, College of Science, Health, Engineering and Education, Murdoch University, 90 South Street, Murdoch, 6150 Western Australia, Australia; Hubei International Scientific and Technological Cooperation Base of Veterinary Epidemiology, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: xiaojie.zhu@murdoch.edu.au.
³ The State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China; Hubei International Scientific and Technological Cooperation Base of Veterinary Epidemiology, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: yuxz19531@163.com.
⁴ Henan Dairy Herd Improvement Center, Zhengzhou 450046, China. Electronic address: lhuan199007@163.com.
⁵ Henan Dairy Herd Improvement Center, Zhengzhou 450046, China. Electronic address: zzgxu@163.com.
⁶ Henan Dairy Herd Improvement Center, Zhengzhou 450046, China. Electronic address: yanleihcy@163.com.
⁷ The State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China; Hubei International Scientific and Technological Cooperation Base of Veterinary Epidemiology, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: chenyingyu@mail.hzau.edu.cn.
⁸ The State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China; School of Veterinary Medicine, College of Science, Health, Engineering and Education, Murdoch University, 90 South Street, Murdoch, 6150 Western Australia, Australia; Hubei International Scientific and Technological Cooperation Base of Veterinary Epidemiology, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: I.Robertson@murdoch.edu.au.
⁹ The State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China; School of Veterinary Medicine, College of Science, Health, Engineering and Education, Murdoch University, 90 South Street, Murdoch, 6150 Western Australia, Australia; Hubei International Scientific and Technological Cooperation Base of Veterinary Epidemiology, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: aizhen@mail.hzau.edu.cn.
¹⁰ School of Veterinary Medicine, College of Science, Health, Engineering and Education, Murdoch University, 90 South Street, Murdoch, 6150 Western Australia, Australia; Centre for Animal Production and Health, Future Foods Institute, Murdoch University, 90 South Street, Murdoch, 6150 WA, Australia. Electronic address: J.Aleri@murdoch.edu.au.

PMID: 36716653
DOI: 10.1016/j.prevetmed.2023.105856

Abstract

Salmonella and ESBL-producing E. coli pose a threat to public health through the food chain. A cross-sectional study was conducted to determine the prevalence and antimicrobial resistance of Salmonella and ESBL E. coli in apparently healthy lactating dairy cattle in Henan Province. Thirty-five lactating cows per farm were sampled by fecal swabbing from 38 farms, with samples being pooled to a total of 7 pooled samples per herd. Eight of the 266 pooled fecal samples (3.0%) were positive for Salmonella (95% confidence intervals (CI): 1.3, 5.8) with a herd-level Salmonella prevalence of 13.2% (95% CI: 4.4, 28.1). The within-herd prevalence for pooled samples for Salmonella ranged from 0.0% to 28.6%. A high proportion of resistance to tetracycline (6/8) and florfenicol (6/8) was obtained in the cultured Salmonella. Multi-drug resistant isolates were observed on 4/5 Salmonella-positive farms. ESBL E. coli were identified on all farms (100% - 34/34, 95% CI: 89.7, 100). All ESBL E. coli isolates (n = 216) contained the bla_CTX-M gene and two isolates also contained the bla_TEM gene. Our study reports the prevalence and antimicrobial resistance of Salmonella and ESBL E. coli in apparently healthy lactating dairy cows from Henan Province.

Keywords: Antimicrobial resistance; Dairy cattle; E. coli; ESBL; Henan; Salmonella.

MeSH terms

Animals
Anti-Bacterial Agents / pharmacology
Cattle
Cattle Diseases* / epidemiology
Cross-Sectional Studies
Drug Resistance, Bacterial
Escherichia coli
Escherichia coli Infections* / epidemiology
Escherichia coli Infections* / veterinary
Female
Lactation
Prevalence
Salmonella
beta-Lactamases / genetics

Substances

Anti-Bacterial Agents
beta-Lactamases