Modeling Distal Middle Cerebral Artery Occlusion in Neonatal Rodents with Magnetic Nanoparticles or Magnetized Red Blood Cells

Jie-Min Jia; Yuxiao Jin

doi:10.1007/978-1-0716-2926-0_7

Modeling Distal Middle Cerebral Artery Occlusion in Neonatal Rodents with Magnetic Nanoparticles or Magnetized Red Blood Cells

Methods Mol Biol. 2023:2616:55-65. doi: 10.1007/978-1-0716-2926-0_7.

Authors

Jie-Min Jia¹, Yuxiao Jin²

Affiliations

¹ Westlake Laboratory of Life Sciences and Biomedicine, School of Life Sciences, Westlake University, Hangzhou, China. jiajiemin@westlake.edu.cn.
² Westlake Laboratory of Life Sciences and Biomedicine, School of Life Sciences, Westlake University, Hangzhou, China.

PMID: 36715928
DOI: 10.1007/978-1-0716-2926-0_7

Abstract

It is challenging to establish animal models to mimic perinatal arterial ischemic stroke. Here, we provided two approaches that precisely occlude rodent pups' distal middle cerebral artery of rodent pups at any postnatal age. One uses magnetic nanoparticles to generate platelet-rich thrombus, and the other utilizes magnetized red blood cells (mRBCs) to generate an erythrocyte-rich embolus. Both approaches result in focal cerebral ischemia followed by controllable reperfusion while requiring no arterial surgery.

Keywords: Magnetic nanoparticle; PAIS; Perinatal stroke; SIMPLE; SIMPLeR; dMCAO; mRBCs.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Brain Ischemia*
Disease Models, Animal
Erythrocytes
Infarction, Middle Cerebral Artery
Magnetite Nanoparticles*
Middle Cerebral Artery
Rodentia
Stroke*

Substances

Magnetite Nanoparticles