Bilateral Carotid Artery Stenosis and Cerebral Blood Flow Outcomes

Ifechukwude Joachim Biose; Gregory Jaye Bix

doi:10.1007/978-1-0716-2926-0_5

Bilateral Carotid Artery Stenosis and Cerebral Blood Flow Outcomes

Methods Mol Biol. 2023:2616:39-46. doi: 10.1007/978-1-0716-2926-0_5.

Authors

Ifechukwude Joachim Biose¹, Gregory Jaye Bix²

Affiliations

¹ Clinical Neuroscience Research Center, Department of Neurosurgery, Tulane University School of Medicine, New Orleans, LA, USA.
² Clinical Neuroscience Research Center, Department of Neurosurgery, Tulane University School of Medicine, New Orleans, LA, USA. gbix@tulane.edu.

PMID: 36715926
DOI: 10.1007/978-1-0716-2926-0_5

Abstract

Bilateral carotid artery stenosis (BCAS) is a valid approach for modeling vascular dementia (VaD) in mice as it induces cerebral hypoperfusion and produces white matter degeneration and cognitive impairment. VaD is one of the major causes of cognitive impairment and currently has no approved therapy; hence its preclinical modeling is warranted for investigating potential therapeutic compounds. BCAS enables the characterization of brain pathology and associated cognitive phenotype of VaD. In this chapter, we describe the surgical method of inducing BCAS in mice, using titanium micro-coils, and we report cerebral blood flow changes before and after surgical induction as well as some histological findings in the corpus callosum of diabetic mice subjected to long-term BCAS.

Keywords: Bilateral carotid artery stenosis; Cerebral hypoperfusion; Vascular dementia; White matter degeneration and cognitive impairment.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Carotid Stenosis* / pathology
Carotid Stenosis* / psychology
Cerebrovascular Circulation / physiology
Cognitive Dysfunction* / etiology
Dementia, Vascular* / etiology
Dementia, Vascular* / pathology
Diabetes Mellitus, Experimental* / complications
Disease Models, Animal
Mice
Mice, Inbred C57BL