Subcortical White Matter Stroke in the Mouse: Inducing Injury and Tracking Cellular Proliferation

Miguel Alejandro Marin; Amy J Gleichman; Andrew J Brumm; S Thomas Carmichael

doi:10.1007/978-1-0716-2926-0_2

Subcortical White Matter Stroke in the Mouse: Inducing Injury and Tracking Cellular Proliferation

Methods Mol Biol. 2023:2616:13-20. doi: 10.1007/978-1-0716-2926-0_2.

Authors

Miguel Alejandro Marin¹, Amy J Gleichman¹, Andrew J Brumm¹, S Thomas Carmichael²

Affiliations

¹ Department of Neurology, David Geffen School of Medicine at University of California - Los Angeles, Los Angeles, CA, USA.
² Department of Neurology, David Geffen School of Medicine at University of California - Los Angeles, Los Angeles, CA, USA. scarmichael@mednet.ucla.edu.

PMID: 36715923
DOI: 10.1007/978-1-0716-2926-0_2

Abstract

Here, we describe a method for inducing subcortical white matter stroke in mice, as well as tracking cellular proliferation through drinking water administration of EdU and ex vivo labeling.

Keywords: 2’-Deoxy-5-ethynyluridine; Cell proliferation; EdU; N5-(1-iminoethyl)-L-ornithine HCL; Vasoconstriction; White matter stroke.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Animals
Cell Proliferation
Hyperplasia / pathology
Mice
Stroke* / pathology
White Matter* / pathology

Abstract

Publication types

MeSH terms

Grants and funding