Estimation of intrinsic water-use efficiency from δ13C signature of C3 leaves: Assumptions and uncertainty

Abstract

Carbon isotope composition (δ¹³C) has been widely used to estimate the intrinsic water-use efficiency (iWUE) of plants in ecosystems around the world, providing an ultimate record of the functional response of plants to climate change. This approach relies on established relationships between leaf gas exchange and isotopic discrimination, which are reflected in different formulations of ¹³C-based iWUE models. In the current literature, most studies have utilized the simple, linear equation of photosynthetic discrimination to estimate iWUE. However, recent studies demonstrated that using this linear model for quantitative studies of iWUE could be problematic. Despite these advances, there is a scarcity of review papers that have comprehensively reviewed the theoretical basis, assumptions, and uncertainty of ¹³C-based iWUE models. Here, we 1) present the theoretical basis of ¹³C-based iWUE models: the classical model (iWUE_sim), the comprehensive model (iWUE_com), and the model incorporating mesophyll conductance (iWUE_mes); 2) discuss the limitations of the widely used iWUE_sim model; 3) and make suggestions on the application of the iWUE_mes model. Finally, we suggest that a mechanistic understanding of mesophyll conductance associated effects and post-photosynthetic fractionation are the bottlenecks for improving the ¹³C-based estimation of iWUE.

Keywords: carbon isotope discrimination; climate change; mesophyll conductance; photosynthesis; post-photosynthetic fractionation; water-use efficiency.