Systematic analysis of photo/sko-regulated germination and post-germination development of shallow photodormant seeds in Nicotiana tabacum L

Qiyuan Liu; Zhenhua Li; Min Zhang; Shuai Dong; Pingping Yang; Jie Zhang; Eddison Loades

doi:10.3389/fpls.2022.1042981

Systematic analysis of photo/sko-regulated germination and post-germination development of shallow photodormant seeds in Nicotiana tabacum L

Front Plant Sci. 2023 Jan 4:13:1042981. doi: 10.3389/fpls.2022.1042981. eCollection 2022.

Authors

Qiyuan Liu¹, Zhenhua Li¹, Min Zhang¹, Shuai Dong¹, Pingping Yang¹, Jie Zhang², Eddison Loades³

Affiliations

¹ College of Agriculture, University of Guizhou, Guiyang, Guizhou, China.
² China National Tobacco Corporation (CNTC) Key Laboratory of Molecular Genetics, Guizhou Academy of Tobacco Science, Guiyang, Guizhou, China.
³ Department of Biological Sciences, Royal Holloway, University of London, London, United Kingdom.

Abstract

Introduction: Light is a major environmental factor in regulating germination and post-germination development of shallow photo-dormant seeds in Nicotiana tabacum L. (tobacco). However, its molecular mechanism remains largely unclear.

Methods and results: In this study, we compared the phenotypes of the seeds germinated under light and dark, and systematically investigated their regulatory networks by integrating transcriptomic and proteomic data. Under light, the germination increased ~25%, the length of the hypocotyl shortened ~3 cm, and the apical hook disappeared. 9, 161, 342 differentially expressed genes (DEGs) and 128, 185, 81 differentially expressed proteins (DEPs) were regulated by light in the development stage of seed imbibition, radicle protrusion and cotyledon expansion respectively. 0, 19 and 1 co-up-regulated and 1, 30 and 64 co-down-regulated DEGs (DEP) were observed in the three stages, respectively. Of them, 2S albumin large chain, was down-regulated by light in imbibed seed. Oleosin 18.5 kDa (OLEO1) and Glyceraldehyde-3-phosphate dehydrogenase (GAPA1), Oxygen-evolving enhancer protein 1-1 and anchloroplastic (PSBO1), hub genes (proteins) in protein-protein interaction network (PPI), were downregulated and up-regulated in germinated seeds by light, respectively. OLEO1, a hub gene (proteins), was down-regulated by light in post-germination seedling.

Conclusion: These results systematically revealed the molecular networks regulated by light during germination and post-germination development of shallow photo-dormant tobacco seeds.

Keywords: cotyledon expansion; imbibition; proteome; radicle protrusion; seed germination; transcriptome.

Grants and funding

This study was supported by the National Natural Science Foundation of China (31860420, 32060512), the Major project of Guizhou Tobacco Company [201904], the Science and Technology Plan Project of Guizhou Province ([2018]5781). The authors declare that this study received funding from Guichou Tobacco Company. The funder was not involved in the study design, collection, analysis, interpretation of data, the writing of this article, or the decision to submit it for publication.