Tandem mass tag-based quantitative proteomics analysis reveals the new regulatory mechanism of progranulin in influenza virus infection

Haoning Li; Yuying Zhang; Chengye Li; Peng Ning; Hailiang Sun; Fanhua Wei

doi:10.3389/fmicb.2022.1090851

Tandem mass tag-based quantitative proteomics analysis reveals the new regulatory mechanism of progranulin in influenza virus infection

Front Microbiol. 2023 Jan 12:13:1090851. doi: 10.3389/fmicb.2022.1090851. eCollection 2022.

Authors

Haoning Li¹, Yuying Zhang², Chengye Li¹, Peng Ning¹, Hailiang Sun³, Fanhua Wei¹

Affiliations

¹ College of Agriculture, Ningxia University, Yinchuan, China.
² School of Biological Science and Technology, University of Jinan, Jinan, China.
³ College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.

Abstract

Progranulin (PGRN) plays an important role in influenza virus infection. To gain insight into the potential molecular mechanisms by which PGRN regulates influenza viral replication, proteomic analyzes of whole mouse lung tissue from wild-type (WT) versus (vs) PGRN knockout (KO) mice were performed to identify proteins regulated by the absence vs. presence of PGRN. Our results revealed that PGRN regulated the differential expression of ALOX15, CD14, CD5L, and FCER1g, etc., and also affected the lysosomal activity in influenza virus infection. Collectively these findings provide a panoramic view of proteomic changes resulting from loss of PGRN and thereby shedding light on the functions of PGRN in influenza virus infection.

Keywords: PGRN; influenza virus; lysosome; multiplex tandem mass tag; proteome.