Cytotoxic hexadepsipeptides and anti-coronaviral 4-hydroxy-2-pyridones from an endophytic Fusarium sp

Shanshan Chang; Biying Yan; Yuchuan Chen; Wuli Zhao; Rongmei Gao; Yuhuan Li; Liyan Yu; Yunying Xie; Shuyi Si; Minghua Chen

doi:10.3389/fchem.2022.1106869

Cytotoxic hexadepsipeptides and anti-coronaviral 4-hydroxy-2-pyridones from an endophytic Fusarium sp

Front Chem. 2023 Jan 12:10:1106869. doi: 10.3389/fchem.2022.1106869. eCollection 2022.

Authors

Affiliation

¹ Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.

Abstract

Three new hexadepsipeptides (1-3), along with beauvericin (4), beauvericin D (5), and four 4-hydroxy-2-pyridone derivatives (6-9) were isolated from the endophytic fungus Fusarium sp. CPCC 400857 that derived from the stem of tea plant. Their structures were determined by extensive 1D and 2D NMR, and HRESIMS analyses. The absolute configuration of hexadepsipeptides were elucidated by the advanced Marfey's method and chiral HPLC analysis. Compounds 4, and 7-9 displayed the cytotoxicity against human pancreatic cancer cell line, AsPC-1 with IC₅₀ values ranging from 3.45 to 29.69 μM, and 7 and 8 also showed the antiviral activity against the coronavirus (HCoV-OC43) with IC₅₀ values of 13.33 and 6.65 μM, respectively.

Keywords: 4-hydroxy-2-pyridone; Fusarium sp.; advanced Marfey′s method; anti-coronavirus activity; hexadepsipeptides.

Grants and funding

This work was supported by the CAMS Innovation Fund for Medical Sciences (CIFMS, 2021-I2M-1–028, and 2022-I2M-2–002), National Natural Science Foundation of China (NNSFC, 81302675, and 82104047).