Identification of the First Sulfobetaine Hydrogel-Binding Peptides via Phage Display Assay

Abstract

Using the M13 phage display, a series of 7- and 12-mer peptides which interact with new sulfobetaine hydrogels are identified. Two peptides each from the 7- and 12-mer peptide libraries bind to the new sulfobetaine hydrogels with high affinity compared to the wild-type phage lacking a dedicated hydrogel binding peptide. This is the first report of peptides binding to zwitterionic sulfobetaine hydrogels and the study therefore opens up the pathway toward new phage or peptide/hydrogel hybrids with high application potential.

Keywords: LLADTTHHRPWT; QQTNWSL; TVNFKLY; hydrogels; peptide binding; phage displays; sulfobetaine; surface modifications.