Construction and use of an infectious cDNA clone to identify aphid vectors and susceptible monocot hosts of the polerovirus barley virus G

Anna Erickson; Jun Jiang; Yen-Wen Kuo; Bryce W Falk

doi:10.1016/j.virol.2023.01.011

Construction and use of an infectious cDNA clone to identify aphid vectors and susceptible monocot hosts of the polerovirus barley virus G

Virology. 2023 Feb:579:178-185. doi: 10.1016/j.virol.2023.01.011. Epub 2023 Jan 19.

Authors

Anna Erickson¹, Jun Jiang², Yen-Wen Kuo², Bryce W Falk²

Affiliations

¹ Department of Plant Pathology, University of California, Davis, CA, 95616, USA. Electronic address: anerickson@ucdavis.edu.
² Department of Plant Pathology, University of California, Davis, CA, 95616, USA.

PMID: 36702063
DOI: 10.1016/j.virol.2023.01.011

Abstract

Since its discovery in 2016, the Polerovirus Barley virus G has been reported in at least nine countries and multiple species of monocot plants. All of these reports have used PCR and/or sequencing based assays to identify BVG, however none have investigated the biology of BVG. In this study we detail the generation of the first infectious cDNA clone of BVG from archived RNA, thereby producing a valuable experimental tool and system for studying BVG biology. Using this system we identified two compatible aphid vectors and confirmed the susceptibility of several monocot plants, and the dicotyledonous plant host Nicotiana benthamiana, to BVG.

Keywords: Aphid vector; Barley virus G; Host susceptibility; Infectious clone; Polerovirus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Aphids*
DNA, Complementary / genetics
Hordeum* / genetics
Luteoviridae* / genetics
Plant Diseases
Plants

Substances

DNA, Complementary