Frequency of HLA alleles and KIR Ligands in Acute Myeloid Leukemia in Indian Cohort

Selma Zenia D'Silva; Anand Bodade; Shalaka Kadam; Manisha Tambe; Sandeep Dhanda; Bhausaheb Bagal; Manju Sengar; Hasmukh Jain; Lingaraj Nayak; Avinash Bonda; Sachin Punatar; Anant Gokarn; Navin Khattry; Meenakshi Singh

doi:10.1007/s12288-022-01550-0

Frequency of HLA alleles and KIR Ligands in Acute Myeloid Leukemia in Indian Cohort

Indian J Hematol Blood Transfus. 2023 Jan;39(1):50-56. doi: 10.1007/s12288-022-01550-0. Epub 2022 Sep 5.

Authors

Selma Zenia D'Silva¹, Anand Bodade², Shalaka Kadam¹, Manisha Tambe¹, Sandeep Dhanda³, Bhausaheb Bagal^{4

5}, Manju Sengar^{4

5}, Hasmukh Jain^{4

5}, Lingaraj Nayak^{4

5}, Avinash Bonda^{4

5}, Sachin Punatar^{5

6}, Anant Gokarn^{5

6}, Navin Khattry^{5

6}, Meenakshi Singh^{1

5}

Affiliations

¹ Scientific Officer "E", Transplant Immunology and Immunogenetics Laboratory, Advanced Centre for Treatment, Research and Education in Cancer, CCE, Tata Memorial Centre, Plot No.1 & 2, Sector 22, Kharghar, Navi Mumbai 410210 India.
² Department of Transfusion Medicine, Tata Memorial Hospital, Mumbai, India.
³ Department of Oncology, St. Jude Children's Research Hospital, Memphis, TN 38105 USA.
⁴ Department of Medical Oncology, Tata Memorial Hospital, Mumbai, India.
⁵ Homi Bhabha National Institute (HBNI), Mumbai, India.
⁶ Advanced Centre for Treatment, Research and Education in Cancer, Bone Marrow Transplant Unit, Tata Memorial Centre, Kharghar, India.

Abstract

Relationship between various combinations of KIR ligands and HLA alleles have been studied in several diseases. The aim of this retrospective study was to estimate the frequency of HLA alleles and KIR ligands among acute myeloid leukemia patients and healthy controls in order to examine the possible association of HLA alleles and KIR ligands with AML. A total of 439 acute myeloid leukemia patients and 1317 unrelated, healthy ethnic Indian controls were included in the study. HLA typing was performed using PCR-SSP. KIR ligands were assigned by using the KIR ligand Calculator. The frequency of HLA alleles and KIR ligands in patients was then compared with the controls. As compared to controls, frequencies of HLA-A*03 and HLA-B*35 were increased in AML patients, whereas, that of HLA-C*03 was decreased. Frequencies of HLA-A*03 and HLA-C*15 were increased in male patients, however, no significant difference was observed in female patients as compared to controls. In the pediatric group, the frequencies of HLA-A*01 was decreased and that of HLA-A*03 and HLA-B*18 were increased, whereas, frequencies of HLA-B*13 was decreased and that of HLA-B*27 was increased in the adult patients. In the haplotype analysis, the frequency of HLA-A*24/B*35/DRB1*15 was increased in overall patients. In adult group, the frequency of HLA-A*01/B*44/DRB1*07 was increased in patients than in controls. No significant association was observed between KIR ligands and susceptibility/ protection to AML. Our results indicate that certain HLA alleles and haplotypes have presumptive positive or negative role in conferring protection/susceptibility to AML.

Supplementary information: The online version contains supplementary material available at 10.1007/s12288-022-01550-0.

Keywords: AML; Association; Cohort; Human leukocyte antigen; India; KIR ligands.

© The Author(s), under exclusive licence to Indian Society of Hematology and Blood Transfusion 2022, Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.