Biochemical and proteomic insights revealed selenium priming induced phosphorus stress tolerance in common bean (Phaseolus vulgaris L.)

Abstract

Background: Mineral stress is one of the dominating abiotic stresses, which leads to decrease in crop production. Selenium (Se) seed priming is a recent approach to mitigate the plant's mineral deficiency stress. Although not an essential element, Se has beneficial effects on the plants in terms of growth, quality, yield and plant defense system thus, enhancing plant tolerance to mineral deficiency.

Methods and results: The present research was accomplished to find out the effect of Se priming on common bean plant (SFB-1 variety) under phosphorus (P) stress. The seeds were grown invitro on four different MGRL media which are normal MGRL media as control with non-Se primed seeds (Se^- P⁺), non -Se primed seeds grown on P deficient MGRL media (Se^- P^-), Se primed seeds grown on normal MGRL media (Se⁺P⁺) and Se primed seeds grown on P deficient MGRL media (Se⁺P ^-). The various morphological and biochemical parameters such as proline content, total sugar content, polyphenols and expression of proteins were analyzed under P stress. The results showed that Se priming has significantly (p ≤ 0.05) affected the morphological as well as biochemical parameters under normal and P stress conditions. The morphological parameters-length, weight, number of nodes and leaves of Se⁺P⁺, Se⁺P^- root and shoot tissue showed significant increase as compared to Se^-P⁺, Se^-P^-. Similarly various biochemical parameters such as total chlorophyll content, proline, total sugar content and polyphenols of Se⁺P⁺, Se⁺P^- increased significantly as compared to Se^-P⁺, Se^-P^-. The differential protein expression in both Se⁺P⁺, Se⁺P^- and Se^-P⁺, Se^-P^- plants were determined using MALDI-MS/MS. The differentially expressed proteins in Se⁺P⁺, Se⁺P^- plants were identified as caffeic acid-3-O-methyltransferase (COMT) and SecA protein (a subunit of Protein Translocan transporter), and are found responsible for lignin synthesis in root cell walls and ATP dependent movement of thylakoid proteins across the membranes in shoot respectively. The differential expression of proteins in plant tissues, validated morphological and biochemical responses such as maintaining membrane integrity, enhanced modifications in cellular metabolism, improved polyphenol activities and expression of defensive proteins against mineral deficiency.

Conclusions: The study provided an understanding of Se application as a potential approach increasing tolerance and yield in crop plants against mineral deficiency.

Keywords: Common bean; MALDI-MS/MS; Phosphorus stress; Priming; Selenium.