Circular RNA circPlce1 regulates innate immune response in miiuy croaker, Miichthys miiuy

Xing Lv; Qiang Luo; Shiying Xin; Weiwei Zheng; Tianjun Xu; Yuena Sun

doi:10.1016/j.fsi.2023.108561

Circular RNA circPlce1 regulates innate immune response in miiuy croaker, Miichthys miiuy

Fish Shellfish Immunol. 2023 Feb:133:108561. doi: 10.1016/j.fsi.2023.108561. Epub 2023 Jan 20.

Authors

Xing Lv¹, Qiang Luo¹, Shiying Xin¹, Weiwei Zheng¹, Tianjun Xu², Yuena Sun³

Affiliations

¹ Laboratory of Fish Molecular Immunology, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China.
² Laboratory of Fish Molecular Immunology, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China; Laboratory of Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China. Electronic address: tianjunxu@163.com.
³ Laboratory of Fish Molecular Immunology, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, China; National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, China. Electronic address: yuenasun@163.com.

PMID: 36690265
DOI: 10.1016/j.fsi.2023.108561

Abstract

In recent years, more and more researchers have devoted to the study of circular RNAs (circRNAs) in noncoding RNAs. As an important regulator in a variety of biological processes, circRNAs are relatively abundant in the study of mammals, while research in lower vertebrates is still lacking. In this study, we found a circRNA, circPlce1, related to innate immune response in Miichthys miiuy (miiuy croaker). The experimental results confirmed that circPlce1 could promote the production of antiviral genes and inflammatory response under the stimulation of poly (I: C) and LPS. We also confirmed that circPlce1 can promote NF-κB and IRF3 pathways through luciferase reporter assay experiment. In addition, we also found that circPlce1 can promote cell proliferation and improve cell viability. In conclusion, our results showed that circPlce1 plays an active role in regulating inflammatory response, cell proliferation and cell viability, providing a foundation for the study of the biological function of circRNAs in the innate immune response in teleost fish.

Keywords: Fish; Immune response; Innate immunity; circRNA.

MeSH terms

Amino Acid Sequence
Animals
Fish Proteins / genetics
Fish Proteins / metabolism
Immunity, Innate / genetics
Mammals / metabolism
Perciformes*
RNA, Circular* / genetics

Substances

RNA, Circular
Fish Proteins