The impact of osmotic stresses on the biofilm formation, immunodetection, and morphology of Aeromonas hydrophila

Wenbin Wang; Ye Cao; Jing Li; Shuaichen Lu; Hongxing Ge; Saikun Pan; Xiaoyi Pan; Luxin Wang

doi:10.1016/j.micres.2023.127301

The impact of osmotic stresses on the biofilm formation, immunodetection, and morphology of Aeromonas hydrophila

Microbiol Res. 2023 Apr:269:127301. doi: 10.1016/j.micres.2023.127301. Epub 2023 Jan 13.

Authors

Wenbin Wang¹, Ye Cao², Jing Li², Shuaichen Lu², Hongxing Ge³, Saikun Pan¹, Xiaoyi Pan⁴, Luxin Wang⁵

Affiliations

¹ Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang, Jiangsu, China; Key Laboratory of Healthy Freshwater Aquaculture, Ministry of Agriculture and Rural Affairs; Key Laboratory of Fish Health and Nutrition of Zhejiang Province; Zhejiang Institute of Freshwater Fisheries, Huzhou, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang, Jiangsu, China; Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang, Jiangsu, China.
² Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang, Jiangsu, China; Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang, Jiangsu, China.
³ Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang, Jiangsu, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang, Jiangsu, China; Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang, Jiangsu, China.
⁴ Key Laboratory of Healthy Freshwater Aquaculture, Ministry of Agriculture and Rural Affairs; Key Laboratory of Fish Health and Nutrition of Zhejiang Province; Zhejiang Institute of Freshwater Fisheries, Huzhou, China. Electronic address: panxiaoyi@163.com.
⁵ Department of Food Science and Technology, University of California Davis, Davis, CA 95616, USA. Electronic address: lxwang@ucdavis.edu.

PMID: 36689842
DOI: 10.1016/j.micres.2023.127301

Abstract

Aeromonas hydrophila (Ah) is a zoonotic pathogen of great importance to aquaculture and human health. This study systematically evaluated the impact of salinity, sugar, ammonia nitrogen, and nitric nitrogen levels on the fitness of Ah by using Luria-Bertani (LB) broth supplemented with different concentrations of NaCl, sucrose, NH₄Cl, urea, NaNO₂ or NaNO₃. Results showed that the static biofilm formation of Ah was higher at 28 °C compared to 37 °C (P < 0.05). At 28 °C, as the NaCl (>1 %) and sucrose levels increased, the Ah biofilm formation and the binding between Ah cells and monoclonal antibodies (mAbs, for immunodetection) decreased. Elevated ammonia nitrogen and nitric nitrogen levels generated no significant impact on Ah biofilm formation or immunodetection (P > 0.05). The expression of mAbs-targeted Omp remained unchanged under high NaCl or sucrose conditions. Further analysis showed that high sucrose conditions led to the over-expression of the extracellular polysaccharides (PS) and promoted the formation of capsule-like structures. These over-expressed PS and capsule structures might be one reason explaining the inhibited immunodetection efficacy. Results generated from this study provide crucial insights for the design of recovery and detection protocols for Ah present in food or environmental samples.

Keywords: Aeromonas hydrophila; Biofilm; Lipopolysaccharides, identification; Osmotic stress.

MeSH terms

Aeromonas hydrophila*
Ammonia / metabolism
Biofilms
Humans
Osmotic Pressure
Sodium Chloride* / metabolism
Sucrose / metabolism

Substances

Sodium Chloride
Ammonia
Sucrose