Determining miRNA Expression Patterns in Xenopus

Marco Antonaci; Alice M Godden; Grant N Wheeler

doi:10.1007/978-1-0716-2982-6_11

Determining miRNA Expression Patterns in Xenopus

Methods Mol Biol. 2023:2630:145-154. doi: 10.1007/978-1-0716-2982-6_11.

Authors

Marco Antonaci^#¹, Alice M Godden^#¹, Grant N Wheeler²

Affiliations

¹ School of Biological Sciences, University of East Anglia, Norwich, UK.
² School of Biological Sciences, University of East Anglia, Norwich, UK. grant.wheeler@uea.ac.uk.

^# Contributed equally.

PMID: 36689182
DOI: 10.1007/978-1-0716-2982-6_11

Abstract

Whole-mount in situ hybridization (WISH) is a technique that enables temporal and spatial visualization of RNA molecules in an embryo or whole tissue by using a complementary labelled probe. MicroRNAs are short noncoding RNAs of 20-25 nt in length mainly involved in posttranscriptional regulation of gene expression. In this chapter, we describe how to visualize miRNAs in Xenopus laevis or tropicalis by WISH using two different approaches: LNA-WISH to visualize mature miRNAs and pri-miRNA-WISH to visualize the immature form of miRNAs, the pri-miRNAs.

Keywords: Development; Expression pattern; WISH; Xenopus; miRNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Gene Expression Regulation
MicroRNAs* / genetics
RNA, Small Untranslated*
Xenopus laevis / metabolism

Substances

MicroRNAs
RNA, Small Untranslated

Grants and funding

BB/M011216/1/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom