Naphthalene is one of the priority pollutants in the environment which can effectively managed through bioremediation approach. Here fungal remediation of PAHs may be cost-effective technology. In present research study, biodegradation of naphthalene at varying concentrations in laboratory scale was investigated using Fusarium proliferatum WC416 isolated from refinery effluent. Degradation of naphthalene was computed by high-performance liquid chromatography (HPLC) and gas chromatography -mass spectroscopy (GC-MS). The results show that Fusarium proliferatum WC416 has effectively degraded the naphthalene to salicylamide and salicylic acid. The highest degradation achieved was 83% for the concentration of 100 ppm followed 74% and 63% for the concentration of 250 ppm and 500 ppm respectively. The intermediates confirmed by GC-MS were salicylic acid (m/z identification 138) and salicylamide (m/z identification 137). During the study, fungal growth, COD, pH, and surface tension have been monitored, which show the continuous variation that indicates progressive biodegradation of naphthalene. The qualitative assessment of extracellular enzymes shows positive activity for lipase, manganese peroxidase, and laccase. The present study also assessed the biosurfactant production by fungus which was characterized as sophorolipid in nature. The study suggests that Fusarium proliferatum WC416 would be an efficient degrader of naphthalene for environmental clean-up.
Keywords: F. proliferatum WC416; Biodegradation; Biosurfactant; Fungi; Naphthalene.
© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.