Mercury sulfide nanoparticles (HgSNP), as natural metal-containing nanoparticles, are the dominant Hg species in anoxic zones. Although the microbial Hg methylation of HgSNP has been previously reported, the importance of this process in Hg methylation has yet to be clarified due to the lack of knowledge on the internalization and transformation of HgSNP. Here, we investigated the internalization and transformation of HgSNP in microbial methylator Geobacter sulfurreducens PCA through total Hg analysis and different Hg species quantification in medium and cytoplasm. We found that the microbial uptake of HgSNP, via a passive diffusion pathway, was significantly higher than that of the Hg2+-dissolved organic matter (Hg2+-DOM) complex. Internalized HgSNP were dissolved to Hg2+ in cytoplasm with a maximal dissolution of 41%, suggesting a "Trojan horse" mechanism. The intracellular Hg2+ from HgSNP exposure at the initial stage (8 h) was higher than that in Hg2+-DOM group, which led to higher methylation of HgSNP. Furthermore, no differences in methylmercury (MeHg) production from HgSNP were observed between the hgcAB gene knockout (ΔhgcAB) and wild-type strains, suggesting that HgSNP methylation may occur through HgcAB-independent pathways. Considering the possibility of a broad range of hgcAB-lacking microbes serving as methylators for HgSNP and the ubiquity of HgSNP in anoxic environments, this study highlights the importance of HgSNP internalization and methylation in MeHg production and demonstrates the necessity of understanding the assimilation and transformation of nutrient and toxic metal nanoparticles in general.
Keywords: bioavailability; hgcAB; internalization; mercury sulfide nanoparticles; methylation.