An integrin binding motif in TLN-1/talin plays a minor role in motility and ovulation

Fereshteh Sadeghian; Ibrahim Ibrahim; Lokesh Ravichandran; Grace Henderson; Anisha Acharya; Lianzijun Wang; Myeongwoo Lee; Erin J Cram

doi:10.17912/micropub.biology.000726

An integrin binding motif in TLN-1/talin plays a minor role in motility and ovulation

MicroPubl Biol. 2023 Jan 4:2023:10.17912/micropub.biology.000726. doi: 10.17912/micropub.biology.000726. eCollection 2023.

Authors

Fereshteh Sadeghian¹, Ibrahim Ibrahim², Lokesh Ravichandran², Grace Henderson², Anisha Acharya², Lianzijun Wang², Myeongwoo Lee², Erin J Cram³

Affiliations

¹ Northeastern University, Department of Bioengineering, Boston, MA, USA.
² Department of Biology, Baylor University, Waco, TX, USA.
³ Northeastern University, Department of Biology, Boston, MA, USA.

Abstract

TLN-1/talin is a conserved focal adhesion protein that forms part of the linkage between the cytoplasmic tail of integrin and the actin cytoskeleton. In C. elegans , TLN-1 is expressed strongly in striated muscle and the gonadal sheath cells. Here, we report that a CRISPR-generated TLN-1 allele TLN-1(W387A), predicted to affect binding of talin to integrins, results in mild phenotypes, including motility defects and ovulation defects. The arrangement of the actin cytoskeleton in the body wall muscles, spermatheca, and sheath appears identical in wild type and TLN-1(W387A) animals. This analysis suggests that W387 in TLN-1 does not have a major effect on the binding of talin to integrin in vivo .