The complete plastome of Centaurium erythraea subsp. majus (Hoffmanns. & Link) M.Laínz (Gentianaceae), the first chloroplast genome belonging to the Centaurium genus

Inês Carvalho Leonardo; Adriana Alberti; France Denoeud; Maria Teresa Barreto Crespo; Jorge Capelo; Frédéric Bustos Gaspar

doi:10.1080/23802359.2022.2160670

The complete plastome of Centaurium erythraea subsp. majus (Hoffmanns. & Link) M.Laínz (Gentianaceae), the first chloroplast genome belonging to the Centaurium genus

Mitochondrial DNA B Resour. 2023 Jan 9;8(1):86-90. doi: 10.1080/23802359.2022.2160670. eCollection 2023.

Authors

Inês Carvalho Leonardo^{1

2}, Adriana Alberti³, France Denoeud³, Maria Teresa Barreto Crespo^{1

2}, Jorge Capelo^{4

5}, Frédéric Bustos Gaspar^{1

2}

Affiliations

¹ iBET, Instituto de Biologia Experimental e Tecnológica, Oeiras, Portugal.
² ITQB-NOVA, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras, Portugal.
³ Génomique Métabolique, Genoscope, Institut François Jacob, CEA, CNRS, Univ Évry, Université Paris-Saclay, Évry, France.
⁴ ECOCHANGE, CIBIO-InBIO - Research Centre in Biodiversity and Genetic Resources, Universidade do Porto, Vairão, Portugal.
⁵ INIAV, Instituto Nacional de Investigação Agrária e Veterinária I.P., Quinta do Marquês, Oeiras, Portugal.

Abstract

Despite having many historically reported ethnomedicinal uses, Centaurium erythraea Rafn (Rafn and Buchs, 1800; common centaury) also produces cytotoxic secondary metabolites, and its presence should be carefully monitored. In this study, the complete chloroplast of Centaurium erythraea subsp. majus (Hoffmanns. & Link) M.Laínz (Laínz, 1971) isolate BPTPS121 is described, being the first available plastome belonging to the Centaurium genus. The chloroplast genome (GenBank accession number: ON641347) is 153,107 bp in length with 37.9% GC content, displaying a quadripartite structure that contains a pair of inverted repeat regions (25,166 bp each), separated by a large single-copy (84,388 bp) and small single-copy (18,387 bp) regions. A total of 129 genes were predicted, including 37 tRNA genes, eight rRNA genes, and 84 protein-coding genes. The phylogenetic analysis showed that isolate BPTPS121 is placed under the Gentianaceae family, belonging to the Gentianales order. The maximum-likelihood tree supports the already described lineage divergence in the Gentianaceae family, with C. erythraea subsp. majus belonging to the Chironieae tribe positioned below the Exaceae tribe and above the Potalieae and the entire Gentianeae tribes. This study will contribute to conservation, phylogenetic, and evolutionary studies, as well as DNA barcoding applications for food, feed, and supplements safety purposes.

Keywords: Centaurium erythraea subsp. majus; Gentianaceae; Illumina MiSeq sequencing; complete chloroplast genome; phylogenetic analysis.

Grants and funding

Conducted under the project ‘barcodingPTplants – DNA barcoding aromatic, medicinal, and condiment plants from Portugal’ [02/SAICT/2017 n° 31074] financed by Programa Lisboa 2020, Portugal 2020, and the European Union, through the European Regional Development Fund (ERDF), and with the support of INCD funded by FCT and ERDF under the project 01/SAICT/2016 n° 022153. Funding from INTERFACE Programme, through the Innovation, Technology and Circular Economy Fund (FITEC), is gratefully acknowledged, as well as iNOVA4Health [UIDB/04462/2020 and UIDP/04462/2020] and LS4FUTURE [LA/P/0087/2020], programs financially supported by FCT-MCTES.