Conditioned medium from human cord blood mesenchymal stem cells attenuates age-related immune dysfunctions

Bo Sun; Xianhui Meng; Yumin Li; Yanlong Li; Rui Liu; Zhongdang Xiao

doi:10.3389/fcell.2022.1042609

Conditioned medium from human cord blood mesenchymal stem cells attenuates age-related immune dysfunctions

Front Cell Dev Biol. 2023 Jan 4:10:1042609. doi: 10.3389/fcell.2022.1042609. eCollection 2022.

Authors

Bo Sun¹, Xianhui Meng¹, Yumin Li¹, Yanlong Li², Rui Liu³, Zhongdang Xiao¹

Affiliations

¹ State key laboratory of bioelectronics, school of biological science and medical engineering, Southeast University, Nanjing, China.
² Shandong Electric Power Central Hospital, Jinan, China.
³ Department of Genetic Engineering, College of Natural Science, University of Suwon, Hwaseong-si, South Korea.

Abstract

Aging is accompanied with progressive deterioration of immune responses and tissue's function. Using 12-month-old mice as model, we showed that conditioned medium of human cord blood mesenchymal stem cells (CBMSC-CM) significantly reduced the population percentage of CD3^-CD335⁺ NK and CD4⁺CD25⁺ regulatory T-cells in peripheral blood. The CBMSC-CM administration also increased naïve T-cells number and restored the ratio of naïve to memory T-cells in CD4⁺ T-cells population. These results indicated that CBMSC-CM improved the immune response efficiency of aged mice. Moreover, we also found CBMSC-CM treatment significantly reduced the number of senescenT-cells in kidney tissues. Finally, we demonstrated that CBMSC-CM remarkably attenuated hydrogen peroxide triggered T-cell response and ameliorated oxidative stress induced cellular senescence. All of these data suggest a prominent anti-aging effect of secretome of CBMSCs.

Keywords: anti-aging; cord blood; immune regulation; mesenchymal stem cells; oxidative damage.