A novel micropattern platform constructed by TiO2 oxidation of PDA

Luying Liu; Peng Ye; Ting Jiang; Sheng Dai; Lei Zhou; Lu Zhang; Jiawei Cui; Zhixing Wang; Jianan Liu; Ping Yang; Jiang Chen

doi:10.1016/j.colsurfb.2023.113141

A novel micropattern platform constructed by TiO₂ oxidation of PDA

Colloids Surf B Biointerfaces. 2023 Mar:223:113141. doi: 10.1016/j.colsurfb.2023.113141. Epub 2023 Jan 11.

Authors

Luying Liu¹, Peng Ye¹, Ting Jiang², Sheng Dai¹, Lei Zhou¹, Lu Zhang¹, Jiawei Cui¹, Zhixing Wang¹, Jianan Liu¹, Ping Yang³, Jiang Chen⁴

Affiliations

¹ Key Laboratory of Advanced Technology for Materials of the Education Ministry, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, PR China.
² School of Life Science And Technology, University of Electronic Science and Technology of China, 610000, PR China.
³ Key Laboratory of Advanced Technology for Materials of the Education Ministry, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, PR China. Electronic address: yangping8@263.net.
⁴ The Department of Ophthalmology, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, No. 32 of the West, PR China. Electronic address: 283876533@qq.com.

PMID: 36682296
DOI: 10.1016/j.colsurfb.2023.113141

Abstract

Dopamine is a small molecule inspired by the dopamine motif of mussel foot proteins, and PDA is formed by the self-polymerization of dopamine. Under the UV-irradiation，PDA would be oxidized by reactive oxygen species (ROS) which were produced by photocatalytic reactions on TiO₂ surfaces，thus regulating the adhesion behavior of endothelial cells (ECs) TiO₂ inhibited platelet (Plt) adhesion after UV exposure. Polydopamine (PDA)-TiO₂ micropatterns (P-PDA-TiO₂) were prepared by magnetron sputtering and photolithography. This micropatterns successfully achieves selective adhesion of Plt and ECs. The selective adhesion of ECs disappears after vacuum reduction. In contrast to conventional cell patterning strategies, P-PDA-TiO₂ can easily achieve pattern separation of ECs and Plts and provide a new concept for building complex blood-contacting devices.

Keywords: ECs; Micropatterning; PDA; Plt; TiO(2).

MeSH terms

Dopamine*
Endothelial Cells*
Polymers / pharmacology

Substances

polydopamine
titanium dioxide
Dopamine
Polymers