Reply to Henriksen, S.; Rinaldo, C.H. Should SVGp12 Be Used for JC Polyomavirus Studies? Comment on "Prezioso et al. COS-7 and SVGp12 Cellular Models to Study JCPyV Replication and MicroRNA Expression after Infection with Archetypal and Rearranged-NCCR Viral Strains. Viruses 2022, 14, 2070"

Carla Prezioso; Ugo Moens; Valeria Pietropaolo

doi:10.3390/v15010093

Reply to Henriksen, S.; Rinaldo, C.H. Should SVGp12 Be Used for JC Polyomavirus Studies? Comment on "Prezioso et al. COS-7 and SVGp12 Cellular Models to Study JCPyV Replication and MicroRNA Expression after Infection with Archetypal and Rearranged-NCCR Viral Strains. Viruses 2022, 14, 2070"

Viruses. 2022 Dec 29;15(1):93. doi: 10.3390/v15010093.

Authors

Carla Prezioso^{1

2}, Ugo Moens³, Valeria Pietropaolo²

Affiliations

¹ IRCCS San Raffaele Roma, Microbiology of Chronic Neuro-Degenerative Pathologies, 00163 Rome, Italy.
² Department of Public Health and Infectious Diseases, Sapienza University of Rome, 00185 Rome, Italy.
³ Department of Medical Biology, Faculty of Health Sciences, University of Tromsø-The Arctic University of Norway, 9037 Tromsø, Norway.

Abstract

In relation to the comment by Henriksen and Rinaldo, the authors intend to emphasize that before every experiment with SVGp12 cells they routinely test the cells for the absence of BKPyV contamination. The scientists can state that the SVGp12 cells used in their laboratory were not infected by BKPyV and that their results were also validated on the COS-7 cell line, which is permissive for JCPyV infection. Therefore, the overall findings of the study and its conclusions remain authentic. The authors recommend the necessity of carefully testing SVGp12 cells for BKPyV infection before use or, alternatively, in case of a first purchase; moreover, it is possible to choose different cell lines to avoid running into this unpleasant situation.

Keywords: BKPyV; JCPyV; SVGp12; contamination; infection; replication.

Publication types

Research Support, Non-U.S. Gov't
Comment

MeSH terms

Animals
BK Virus* / genetics
COS Cells
Chlorocebus aethiops
JC Virus* / genetics
MicroRNAs*

Substances

MicroRNAs

Grants and funding

Carla Prezioso: Italian Ministry of Health (starting grant: SG-2018-12366194); Valeria Pietropaolo: funding acquisition (MIUR Research Grant: RP12117A0F451274).