Recent Developments in DNA-Nanotechnology-Powered Biosensors for Zika/Dengue Virus Molecular Diagnostics

Goeun Park; Hanbin Park; Sang-Chan Park; Moonbong Jang; Jinho Yoon; Jae-Hyuk Ahn; Taek Lee

doi:10.3390/nano13020361

Recent Developments in DNA-Nanotechnology-Powered Biosensors for Zika/Dengue Virus Molecular Diagnostics

Nanomaterials (Basel). 2023 Jan 16;13(2):361. doi: 10.3390/nano13020361.

Authors

Goeun Park¹, Hanbin Park¹, Sang-Chan Park², Moonbong Jang¹, Jinho Yoon³, Jae-Hyuk Ahn², Taek Lee^{1

4}

Affiliations

¹ Department of Chemical Engineering, Kwangwoon University, Seoul 01897, Republic of Korea.
² Department of Electronics Engineering, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Republic of Korea.
³ Department of Biomedical-Chemical Engineering, The Catholic University of Korea, 43 Jibong-ro, Wonmi-gu, Bucheon-si 14662, Gyeonggi-do, Republic of Korea.
⁴ TL Bioindustry, 20 Kwangwoon-ro, Nowon-gu, Seoul 01897, Republic of Korea.

Abstract

Zika virus (ZIKV) and dengue virus (DENV) are highly contagious and lethal mosquito-borne viruses. Global warming is steadily increasing the probability of ZIKV and DENV infection, and accurate diagnosis is required to control viral infections worldwide. Recently, research on biosensors for the accurate diagnosis of ZIKV and DENV has been actively conducted. Moreover, biosensor research using DNA nanotechnology is also increasing, and has many advantages compared to the existing diagnostic methods, such as polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA). As a bioreceptor, DNA can easily introduce a functional group at the 5' or 3' end, and can also be used as a folded structure, such as a DNA aptamer and DNAzyme. Instead of using ZIKV and DENV antibodies, a bioreceptor that specifically binds to viral proteins or nucleic acids has been fabricated and introduced using DNA nanotechnology. Technologies for detecting ZIKV and DENV can be broadly divided into electrochemical, electrical, and optical. In this review, advances in DNA-nanotechnology-based ZIKV and DENV detection biosensors are discussed.

Keywords: DENV; DNA nanotechnology; ZIKV; electrical biosensor; electrochemical biosensor; optical biosensor.

Publication types

Review

Grants and funding

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIT) (no. 2021R1C1C1005583) and by the Industrial Core Technology Development Program (20009121, Development of early diagnostic system of peritoneal fibrosis by multiplex detection of exosomal nucleic acids and protein markers) funded by the Ministry of Trade, Industry and Energy (MOTIE, Korea), by the excellent researcher support project of Kwangwoon University in 2022, and finally, this work was supported by the National Research Foundation of Korea Grant funded by the Korean Government (MOE).