FNDC5 Promotes Adipogenic Differentiation of Primary Preadipocytes in Mashen Pigs

Genes (Basel). 2022 Dec 28;14(1):90. doi: 10.3390/genes14010090.

Abstract

Fibronectin type III domain-containing protein 5 (FNDC5) plays an important role in fat deposition, which can be cut to form Irisin to promote fat thermogenesis, resulting in a decrease in fat content. However, the mechanism of FNDC5 related to fat deposition in pigs is still unclear. In this research, we studied the expression of FNDC5 on different adiposes and its function in the adipogenic differentiation of primary preadipocytes in Mashen pigs. The expression pattern of FNDC5 was detected by qRT-PCR and Western blotting in Mashen pigs. FNDC5 overexpression and interference vectors were constructed and transfected into porcine primary preadipocytes by lentivirus. Then, the expression of key adipogenic genes was detected by qRT-PCR and the content of lipid droplets was detected by Oil Red O staining. The results showed that the expression of FNDC5 in abdominal fat was higher than that in back subcutaneous fat in Mashen pigs, whereas the expression in back subcutaneous fat of Mashen pigs was significantly higher than that of Large White pigs. In vitro, FNDC5 promoted the adipogenic differentiation of primary preadipocytes of Mashen pigs and upregulated the expression of genes related to adipogenesis, but did not activate the extracellular signal-regulated kinase (ERK) signaling pathway. This study can provide a theoretical basis for FNDC5 in adipogenic differentiation in pigs.

Keywords: ERK1/2; FNDC5; Mashen pigs; adipogenic differentiation; primary preadipocytes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abdominal Fat / metabolism
  • Adipocytes* / metabolism
  • Adipogenesis* / genetics
  • Animals
  • Fibronectins / genetics
  • Subcutaneous Fat
  • Swine
  • Transcription Factors / metabolism

Substances

  • Fibronectins
  • Transcription Factors

Grants and funding

This research was funded by the National Natural Science Foundation of China (32272846; 31872336), the National Agricultural Development Area Doctor Workstation Project (JZNGQBSGZZ005), the Basic Research Project of Shanxi Province (201901D211376; 201901D211369), the Shanxi Agricultural University Science and Technology Innovation Fund (2018YJ35) and the Shanxi Province Outstanding Doctor Award Fund (SXYBKY2018012).