The radical scavenging activity of three molecular antioxidants (trolox, rutin and ellagic acid) was investigated in different solvents with and without added polymer-based colloidal particles (SL-IP-2). Rutin and ellagic acid showed poor solubility in water, preventing the accurate measurement of the effective antioxidant concentration values, which were determined in ethanol/water (EtOH/H2O) mixtures. The presence of trolox and rutin changed neither the surface charge properties nor the size of SL-IP-2 in these solvents, while significant adsorption on SL-IP-2 was observed for ellagic acid leading to overcharging and rapid particle aggregation at appropriately high antioxidant concentrations in EtOH/H2O. The differences in the radical scavenging capacity of trolox and ellagic acid that was observed in homogeneous solutions using water or EtOH/H2O as solvents vanished in the presence of the particles. Rutin lost its activity after addition of SL-IP-2 due to the larger molecular size and lower exposure of the functional groups to the substrate upon interaction with the particles. The obtained results shed light on the importance of the type of solvent and particle-antioxidant interfacial effects on the radical decomposition ability of molecular antioxidants, which is of crucial importance in industrial processes involving heterogeneous systems.
Keywords: DPPH; colloidal dispersion; latex; molecular antioxidants; solubility.