The ferroptosis in haemocytes of Pacific oyster Crassostrea gigas upon erastin treatment

Zhicheng Guo; Jiejie Sun; Xiaoqian Lv; Tong Zhang; Hongsheng Yao; Wei Wu; Zhen Xing; Ning Kong; Lingling Wang; Linsheng Song

doi:10.1016/j.fsi.2023.108556

The ferroptosis in haemocytes of Pacific oyster Crassostrea gigas upon erastin treatment

Fish Shellfish Immunol. 2023 Feb:133:108556. doi: 10.1016/j.fsi.2023.108556. Epub 2023 Jan 18.

Authors

Affiliations

¹ Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, 116023, China; Liaoning Key Laboratory of Marine Animal Immunology & Disease Control, Dalian Ocean University, Dalian, 116023, China.
² Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, 116023, China; Laboratory of Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266235, China; Liaoning Key Laboratory of Marine Animal Immunology & Disease Control, Dalian Ocean University, Dalian, 116023, China. Electronic address: wanglingling@dlou.edu.cn.
³ Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, 116023, China; Laboratory of Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266235, China; Liaoning Key Laboratory of Marine Animal Immunology & Disease Control, Dalian Ocean University, Dalian, 116023, China.

PMID: 36669600
DOI: 10.1016/j.fsi.2023.108556

Abstract

Ferroptosis is an iron and oxidative dependent form of cell death usually mediated by redox related molecules in vertebrates. In the present study, a glutathione peroxidase 4 (GPX4) and a solute carrier family 7 member 11 (SLC7A11, xCT) homologues were identified from the oyster Crassostrea gigas (designed as CgGPX4 and CgxCT), which contained a GSHPx domain and an AA_permease domain, respectively. The mRNA transcripts of CgGPX4 and CgxCT were expressed in all the examined tissues, including gill, gonad, adductor muscle, labial palp, mantle, hepatopancreas and haemocytes, with the highest expression in haemocytes. After erastin treatment, the rate of cell malformation and cell death increased significantly in haemocytes, and the mitochondrial atrophy, crest loss and fracture were observed in haemocytes. While the amount of Fe²⁺ and Malondialdehyde (MDA) increased significantly, the mRNA expressions of CgGPX4, CgxCT and voltage-dependent anion channel 2 (CgVDAC2) in haemocytes decreased significantly after erastin treatment. These results indicated that erastin was able to induce the ferroptosis of oyster haemocytes.

Keywords: Crassostrea gigas; Erastin; Ferroptosis; Haemocytes; Mitochondria.

MeSH terms

Animals
Carrier Proteins / metabolism
Crassostrea* / metabolism
Ferroptosis*
Hemocytes / metabolism
RNA, Messenger / metabolism

Substances

erastin
Carrier Proteins
RNA, Messenger