Ultrasensitive Lateral Flow Immunoassay for Fumonisin B1 Detection Using Highly Luminescent Aggregation-Induced Emission Microbeads

Ge Xu; Xiaojing Fan; Xirui Chen; Zilong Liu; Guoxin Chen; Xiaxia Wei; Xiangmin Li; Yuankui Leng; Yonghua Xiong; Xiaolin Huang

doi:10.3390/toxins15010079

Ultrasensitive Lateral Flow Immunoassay for Fumonisin B1 Detection Using Highly Luminescent Aggregation-Induced Emission Microbeads

Toxins (Basel). 2023 Jan 16;15(1):79. doi: 10.3390/toxins15010079.

Authors

Affiliations

¹ State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Nanchang University, Nanchang 330047, China.
² School of Future Technology, Nanchang University, Nanchang 330047, China.
³ School of Food Science and Engineering, Hainan University, Haikou 570228, China.

Abstract

Lateral flow immunoassay (LFIA) based on fluorescent microbeads has attracted much attention for its use in rapid and accurate food safety monitoring. However, conventional fluorescent microbeads are limited by the aggregation-caused quenching effect of the loaded fluorophores, thus resulting in low signal intensity and insufficient sensitivity of fluorescent LFIA. In this study, a green-emitting fluorophore with an aggregation-induced emission (AIE) characteristic was encapsulated in polymer nanoparticles via an emulsification technique to form ultrabright fluorescent microbeads (denoted as AIEMBs). The prepared AIEMBs were then applied in a competitive LFIA (AIE-LFIA) as signal reporters for the rapid and highly sensitive screening of fumonisin B1 (FB1) in real corn samples. High sensitivity with a detection limit of 0.024 ng/mL for FB1 was achieved by the developed AIE-LFIA. Excellent selectivity, good accuracy, and high reliability of the AIE-LFIA were demonstrated, indicating a promising platform for FB1 screening.

Keywords: aggregation-induced emission; fluorescent microbeads; fumonisin B1; lateral flow immunoassay.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Gold*
Immunoassay / methods
Limit of Detection
Metal Nanoparticles*
Microspheres
Reproducibility of Results

Substances

fumonisin B1
Gold

Grants and funding

This work was supported by the National Natural Science Foundation, China (No. 32172296, 32160599, 32160598), and Jiangxi Provincial Natural Science Foundation (No. 20212BAB205032, 20192BAB204021).