Effects of Bacillus amyloliquefaciens XJ-BV2007 on Growth of Alternaria alternata and Production of Tenuazonic Acid

Toxins (Basel). 2023 Jan 7;15(1):53. doi: 10.3390/toxins15010053.

Abstract

Large amounts of processing tomato are grown in Xinjiang, China. Tomato black spot disease, caused by Alternaria spp., and the produced alternaria toxins in tomato products are posing risks to human health. In this study, we isolated a rhizospheric bacterium, XJ-BV2007, from tomato (Solanum lycopersicum) fields, which we identified as Bacillus amyloliquefaciens. We found that this bacterium has a strong antagonistic effect against Alternaria alternata and reduces the accumulation of alternaria toxins in tomatoes. According to the antifungal activity of the bacteria-free filtrate, we revealed that B. amyloliquefaciens XJ-BV2007 suppresses A. alternata by the production of antifungal metabolites. Combining semi-preparative high-performance liquid chromatography, we employed UPLC-QTOF-MS analysis and the Oxford cup experiment to find that fengycin plays an important role in inhibiting A. alternata. This paper firstly reported that B. amyloliquefaciens efficiently controls tomato black spot disease and mycotoxins caused by A. alternata. B. amyloliquefaciens XJ-BV2007 may provide an alternative biocontrol strain for the prevention of tomato black spot disease.

Keywords: Alternaria alternata; Bacillus amyloliquefaciens; fengycin; lipopeptide; tenuazonic acid; tomato.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternaria / metabolism
  • Antifungal Agents / metabolism
  • Antifungal Agents / pharmacology
  • Bacillus amyloliquefaciens* / metabolism
  • Humans
  • Lactones / analysis
  • Solanum lycopersicum*
  • Tenuazonic Acid / analysis
  • Toxins, Biological* / metabolism

Substances

  • Tenuazonic Acid
  • Antifungal Agents
  • Lactones
  • Toxins, Biological

Supplementary concepts

  • Alternaria alternata

Grants and funding

This research was funded by the National Key Research and Development Program of China (2019YFC1604502), Natural Science Foundation (2022D01A89) and agricultural science and technology extension and service project (NTFW-2022-14) of Xinjiang Uygur Autonomous Region, National Natural Science Foundation of China (32260634), and Science and Technology Department of Xinjiang Uygur Autonomous Region (2021A02002-4).