Enzymatic Sequencing of Heparin Oligosaccharides Using Exolyase

Qingdong Zhang; Danrong Lu; Fuchuan Li

doi:10.1007/978-1-0716-2946-8_18

Enzymatic Sequencing of Heparin Oligosaccharides Using Exolyase

Methods Mol Biol. 2023:2619:249-256. doi: 10.1007/978-1-0716-2946-8_18.

Authors

Qingdong Zhang¹, Danrong Lu¹, Fuchuan Li²

Affiliations

¹ School of Life Science and Technology, Weifang Medical University, Weifang, China.
² National Glycoengineering Research Center and Shandong Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, Qingdao, China. fuchuanli@sdu.edu.cn.

PMID: 36662475
DOI: 10.1007/978-1-0716-2946-8_18

Abstract

Heparin/heparan sulfate (HP/HS) is a class of acidic polysaccharides with many potential medical applications, especially HP, and its derivatives, low molecular weight heparins (LMWHs), have been widely used as anticoagulants to treat thrombosis for decades. However, the complex structure endows HP/HS a variety of biological functions and hinders the structural and functional studies of HP/HS. Heparinases derived from bacteria are useful tools for the structural studies of HP/HS as well as the preparation of LMWHs. The enzymatic method for the structural analysis of HP/HS chains is easy to operate, requires less samples, and is low cost. Here, we describe an enzymatic approach to investigate the primary sequences of the HP/HS oligosaccharides using a recently discovered exotype heparinase.

Keywords: Enzymatic sequencing; Exotype heparinase; Heparan sulfate; Heparin; Oligosaccharides.

MeSH terms

Anticoagulants
Heparin Lyase
Heparin* / chemistry
Heparitin Sulfate* / chemistry
Oligosaccharides / chemistry

Substances

Heparin
Heparitin Sulfate
Heparin Lyase
Anticoagulants
Oligosaccharides