Honey is a complex mixture, containing ~180 compounds, produced by the Apis melifera bees, with promising antimicrobial and antioxidant properties. Nevertheless, the mechanisms through which honey exerts its effects remain under investigation. Plant antioxidants are found in honey and other bee products exhibiting a high bioactivity and molecular diversity. The aim of the present study was to estimate the antioxidant capacity of honey collected from areas in Greece by small‑scale producers by i) using in vitro cell free assays; and ii) by investigating the effects of honey varieties on the redox status of a liver cancer cell line (HepG2) using non‑cytotoxic concentrations. The findings of the present study will allow for the identification of Greek honeys with promising antioxidant capacity. For this purpose, six types of honey with various floral origins were examined in cell‑free assays followed by cell‑based techniques using flow cytometric analysis and redox biomarker level determination in order to evaluate the potential alterations in the intracellular redox system. The results indicated various mechanisms of action that are dependent on the honey type, concentration dependency and high antioxidant capacity. The extended findings from the literature confirm the ability of raw honey to influence the redox status of HepG2 cells. Nevertheless additional investigations are required to elucidate their mechanisms of action in cell line models.
Keywords: antioxidant activity; cell line; functional food; honey; oxidative stress.