Epitope tag-specific differences in the detection of COSA-1 marked crossover sites in C. elegans spermatocytes

Cori K Cahoon; Celja J Uebel; Anne M Villeneuve; Diana E Libuda

doi:10.17912/micropub.biology.000724

Epitope tag-specific differences in the detection of COSA-1 marked crossover sites in C. elegans spermatocytes

MicroPubl Biol. 2023 Jan 6:2023:10.17912/micropub.biology.000724. doi: 10.17912/micropub.biology.000724. eCollection 2023.

Authors

Cori K Cahoon¹, Celja J Uebel², Anne M Villeneuve², Diana E Libuda¹

Affiliations

¹ Institute of Molecular Biology, Department of Biology, University of Oregon, Eugene, OR, USA.
² Stanford University School of Medicine, Departments of Developmental Biology and Genetics, Stanford, CA, USA.

Abstract

Nascent crossover sites in C. elegans meiocytes can be cytologically detected using epitope-tagged versions of the pro-crossover protein COSA-1. In spermatocytes, differences exist between cytologically-detected and genetically-detected double crossover rates. Here, we examine nascent crossovers using both GFP- and OLLAS-tagged COSA-1. Similar to previous work, we find that most late pachytene spermatocytes display 5 COSA-1 foci, indicating one crossover per autosome bivalent. However, we detected more nuclei with >5 COSA-1 foci using OLLAS::COSA-1, reflecting some bivalents having 2 COSA-1 foci. These results demonstrate tag-specific differences in the detection of COSA-1 marked nascent crossovers in spermatocytes.

Abstract

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