Gene editing nuclease and its application in tilapia

Minghui Li; Deshou Wang

doi:10.1016/j.scib.2017.01.003

Gene editing nuclease and its application in tilapia

Sci Bull (Beijing). 2017 Feb 15;62(3):165-173. doi: 10.1016/j.scib.2017.01.003. Epub 2017 Jan 4.

Authors

Minghui Li¹, Deshou Wang²

Affiliations

¹ Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education, China), Key Laboratory of Aquatic Science of Chongqing, School of Life Sciences, Southwest University, Chongqing 400715, China.
² Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education, China), Key Laboratory of Aquatic Science of Chongqing, School of Life Sciences, Southwest University, Chongqing 400715, China. Electronic address: wdeshou@swu.edu.cn.

PMID: 36659401
DOI: 10.1016/j.scib.2017.01.003

Abstract

Gene editing nucleases including zinc-finger nucleases (ZFNs), transcription activator like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated (Cas) system (CRISPR/Cas9) provide powerful tools that improve our ability to understand the physiological processes and their underlying mechanisms. To date, these approaches have already been widely used to generate knockout and knockin models in a large number of species. Fishes comprise nearly half of extant vertebrate species and provide excellent models for studying many aspects of biology. In this review, we present an overview of recent advances in the use of gene editing nucleases for studies of fish species. We focus particularly on the use of TALENs and CRISPR/Cas9 genome editing for studying sex determination in tilapia.

Keywords: Gene editing nuclease; Genome engineering; Sex determination; Tilapia.

Publication types

Review