Selection and electrochemical-sensor application of an DNA-aptamer for methyl parathion detection

Anal Chim Acta. 2023 Feb 8:1241:340780. doi: 10.1016/j.aca.2023.340780. Epub 2023 Jan 2.

Abstract

An aptamer-based electrochemical sensor for methyl parathion (MP) detection is herein reported. The modified magnetic beads-systematic evolution of ligands by enrichment (MB-SELEX) was used to select the MP aptamer. After 14 rounds of selection, the aptamer (MPapta-6) with high affinity for MP was obtained, and its dissociation constant (Kd) was 39.66 ± 14.73 μM. Using the MPapta-6, the ultra-sensitive electrochemical sensor based on PLL-BP and AuNPs was constructed. The linear range of MP was 1-105 pM and detection limit (LOD) was as low as 0.49 pM. In addition, the application of the sensor in water samples was verified, and the recovery rate was 96.6%-103.5%. The results from this study showed that this strategy could be applied in practical detection.

Keywords: Aptamer; Black phosphorus; Electrochemical aptasensor; Methyl parathion; SELEX.

MeSH terms

  • Aptamers, Nucleotide*
  • Biosensing Techniques* / methods
  • DNA
  • Electrochemical Techniques / methods
  • Gold
  • Limit of Detection
  • Metal Nanoparticles*
  • Methyl Parathion*
  • SELEX Aptamer Technique

Substances

  • Aptamers, Nucleotide
  • Methyl Parathion
  • Gold
  • DNA