Immunoexpression of stem cell markers SOX-2, NANOG AND OCT4 in ameloblastoma

Karolyny Martins Balbinot; Felippe José Almeida Loureiro; Giordanna Pereira Chemelo; Ricardo Alves Mesquita; Aline Maria Pereira Cruz Ramos; Rommel Thiago Jucá Ramos; Artur Luiz da Costa da Silva; Sílvio Augusto Fernandes de Menezes; Maria Sueli da Silva Kataoka; Sergio de Melo Alves Junior; João de Jesus Viana Pinheiro

doi:10.7717/peerj.14349

Immunoexpression of stem cell markers SOX-2, NANOG AND OCT4 in ameloblastoma

PeerJ. 2023 Jan 13:11:e14349. doi: 10.7717/peerj.14349. eCollection 2023.

Affiliations

¹ Laboratory of Pathological Anatomy and Immunohistochemistry, Federal University of Pará, Belém, Pará, Brazil.
² Cell Cultivation Laboratory, Federal University of Pará, Belém, Pará, Brazil.
³ Department of Oral Surgery and Pathology, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
⁴ Health Science Institute, Federal University of Pará, Belém, Pará, Brazil.
⁵ Biological Engineer Laboratory, Park of Science and Technology, Belém, Pará, Brazil.
⁶ Department of Periodontics, Pará State University, Belém, Pará, Brazil.

Abstract

Background: Ameloblastoma (AME) is characterized by a locally invasive growth pattern. In an attempt to justify the aggressiveness of neoplasms, the investigation of the role of stem cells has gained prominence. The SOX-2, NANOG and OCT4 proteins are important stem cell biomarkers.

Methodology: To verify the expression of these proteins in tissue samples of AME, dentigerous cyst (DC) and dental follicle (DF), immunohistochemistry was performed and indirect immunofluorescence were performed on the human AME (AME-hTERT) cell line.

Results: Revealed expression of SOX-2, NANOG and OCT4 in the tissue samples and AME-hTERT lineage. Greater immunostaining of the studied proteins was observed in AME compared to DC and DF (p < 0.001).

Conclusions: The presence of biomarkers indicates a probable role of stem cells in the genesis and progression of AME.

Keywords: Ameloblastoma; Immunohistochemistry; Octamer transcription factors; SOX transcription factors; Stem cells; Transcriptomic.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Ameloblastoma* / genetics
Ameloblastoma* / metabolism
Biomarkers / metabolism
Biomarkers, Tumor / genetics
Biomarkers, Tumor / metabolism
Cell Proliferation
Humans
Immunohistochemistry
Nanog Homeobox Protein / genetics
Neoplastic Stem Cells* / metabolism
Octamer Transcription Factor-3 / genetics
Octamer Transcription Factor-3 / metabolism
SOXB1 Transcription Factors / genetics
SOXB1 Transcription Factors / metabolism
Stem Cells / metabolism

Substances

Biomarkers, Tumor
Nanog Homeobox Protein
NANOG protein, human
Biomarkers
SOXB1 Transcription Factors
Octamer Transcription Factor-3

Associated data

figshare/10.6084/m9.figshare.19517200.v1
figshare/10.6084/m9.figshare.19517263.v2
figshare/10.6084/m9.figshare.21507090.v1
figshare/10.6084/m9.figshare.21507096.v2
figshare/10.6084/m9.figshare.21507102.v1
figshare/10.6084/m9.figshare.21507117.v1

Grants and funding

The authors received funding from the National Council for Scientific and Technological Development, CNPq (Grants #305493/2018-3 and #435644/2018-1 awarded to Ricardo Alves Mesquita; Grant 307584/2018-6 awarded to Rommel T J Ramos; and Grant #429423/2018-7 awarded to João de Jesus Viana Pinheiro). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.