Rapid detection of duck ingredient in adulterated foods by isothermal recombinase polymerase amplification assays

Cang Zhou; Jinfeng Wang; Jialin Xiang; Qi Fu; Xiaoxia Sun; Libing Liu; Lianfeng Ai; Jianchang Wang

doi:10.1016/j.fochms.2023.100162

Rapid detection of duck ingredient in adulterated foods by isothermal recombinase polymerase amplification assays

Food Chem (Oxf). 2023 Jan 9:6:100162. doi: 10.1016/j.fochms.2023.100162. eCollection 2023 Jul 30.

Authors

Cang Zhou^{1

2

3}, Jinfeng Wang², Jialin Xiang², Qi Fu², Xiaoxia Sun², Libing Liu², Lianfeng Ai^{1

2

3}, Jianchang Wang^{1

2

3}

Affiliations

¹ School of Public Health, Hebei Medical University, Shijiazhuang 050017, China.
² Food Microbiology and Animal Quarantine Laboratory, Technology Center of Shijiazhuang Customs, Shijiazhuang 050051, China.
³ Hebei Key Laboratory of Environment and Human Health, Shijiazhuang 050017, China.

Abstract

Duck is often used in meat fraud as a substitute for more expensive meats. Rapid detection of duck ingredient in meat products is of great significance for combating meat fraud and safeguarding the interests of consumers. Therefore, we aim to develop duck-specific recombinase polymerase amplification (RPA)-based assays for the rapid detection of duck ingredient in animal-derived foods. Using Cytb gene as target, the real-time RPA and RPA combined with lateral flow strips (LFS RPA) were developed successfully for the rapid detection of ducks in 20 min at 39 °C and 40 °C, respectively. The assays did not show cross-reactions with 6 other livestock and poultry. The developed RPA assays could detect 10 pg duck genomic DNA per reaction and 0.1 % (w/w) duck ingredient in duck and mutton mixed powder within 30 min, including a rapid nucleic acid extraction. Furthermore, duck ingredient could be detected in 30 different actual foods including heat-processed meats and blood products. Therefore, duck-specific real-time RPA and LFS RPA assays were successfully developed with good specificity and sensitivity, which could enable rapid detection of duck ingredient in the field and provide technical support for combating the meat fraud.

Keywords: Cytb gene; Duck ingredient; LFS RPA; Meat fraud; Rapid detection; Real-time RPA.