Rapid Analysis of Plasmalogen Individual Species by High-Resolution Mass Spectrometry

Glenda Vasku; Niyazi Acar; Olivier Berdeaux

doi:10.1007/978-1-0716-2966-6_22

Rapid Analysis of Plasmalogen Individual Species by High-Resolution Mass Spectrometry

Methods Mol Biol. 2023:2625:259-267. doi: 10.1007/978-1-0716-2966-6_22.

Authors

Glenda Vasku^{1

2}, Niyazi Acar³, Olivier Berdeaux^{1

2}

Affiliations

¹ Eye and Nutrition Research Group, Centre des Sciences du Goût et de l'Alimentation, CNRS, INRAE, Institut Agro, Université de Bourgogne Franche-Comté, Dijon, France.
² PROBE Research Infrastructure, ChemoSens Facility, Dijon, France.
³ Eye and Nutrition Research Group, Centre des Sciences du Goût et de l'Alimentation, CNRS, INRAE, Institut Agro, Université de Bourgogne Franche-Comté, Dijon, France. niyazi.acar@inrae.fr.

PMID: 36653649
DOI: 10.1007/978-1-0716-2966-6_22

Abstract

Analysis and quantification of ether-lipid phospholipid species-also known as plasmalogens-is a crucial step in the study of the biological functions played by these lipids. Application of analytical separation methods and high-resolution mass spectrometry has gained much attention in this regard, while resolution issues and time-consuming sequences interfered with these advances. Herein, we describe a simple and rapid method for the analysis of plasmalogen (Pl) species by HILIC-HRMS. This method is able to identify and quantify relative levels of ethanolamine-plasmalogens (PlsEtn) and choline-plasmalogens (PlsCho) in biological matrices such as whole blood, plasma, erythrocytes, and also retina. Moreover, we provide a detailed and modified lipid extraction method that is applicable to almost all biological matrices.

Keywords: Annotation; Hydrophilic interaction liquid chromatography (HILIC); Lipid extraction; Mass spectrometry (MS); Plasmalogens (Pls).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Plasmalogens* / analysis
Tandem Mass Spectrometry* / methods

Substances

Plasmalogens