Chromosome-level genome assembly of the Colorado potato beetle, Leptinotarsa decemlineata

Junjie Yan; Chaowei Zhang; Mengdi Zhang; Hang Zhou; Zhangqi Zuo; Xinhua Ding; Runzhi Zhang; Fei Li; Yulin Gao

doi:10.1038/s41597-023-01950-5

Chromosome-level genome assembly of the Colorado potato beetle, Leptinotarsa decemlineata

Sci Data. 2023 Jan 19;10(1):36. doi: 10.1038/s41597-023-01950-5.

Authors

Junjie Yan^#¹, Chaowei Zhang^#², Mengdi Zhang¹, Hang Zhou², Zhangqi Zuo², Xinhua Ding³, Runzhi Zhang⁴, Fei Li⁵, Yulin Gao⁶

Affiliations

¹ State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
² State Key Laboratory of Rice Biology & Ministry of Agricultural and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insects & Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Insect Sciences, Zhejiang University, Hangzhou, 310058, China.
³ Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences, Urumqi, 830091, China.
⁴ Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.
⁵ State Key Laboratory of Rice Biology & Ministry of Agricultural and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insects & Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Insect Sciences, Zhejiang University, Hangzhou, 310058, China. lifei18@zju.edu.cn.
⁶ State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. gaoyulin@caas.cn.

^# Contributed equally.

Abstract

The Colorado potato beetle (Leptinotarsa decemlineata) is one of the most notorious insect pests of potatoes globally. Here, we generated a high-quality chromosome-level genome assembly of L. decemlineata using a combination of the PacBio HiFi sequencing and Hi-C scaffolding technologies. The genome assembly (-1,008 Mb) is anchored to 18 chromosomes (17 + XO), with a scaffold N50 of 58.32 Mb. It contains 676 Mb repeat sequences and 29,606 protein-coding genes. The chromosome-level genome assembly of L. decemlineata provides in-depth knowledge and will be a helpful resource for the beetle and invasive biology research communities.

Publication types

Dataset

MeSH terms

Animals
Chromosomes
Coleoptera* / genetics
Genome, Insect*