Honeycomb resin-based spin-column solid-phase extraction for efficient determination of alectinib and its metabolite in human urine

Zihui Zhao; Pengfei Li; Yehong Han; Hongyuan Yan

doi:10.1016/j.chroma.2023.463787

Honeycomb resin-based spin-column solid-phase extraction for efficient determination of alectinib and its metabolite in human urine

J Chromatogr A. 2023 Feb 8:1690:463787. doi: 10.1016/j.chroma.2023.463787. Epub 2023 Jan 10.

Authors

Zihui Zhao¹, Pengfei Li², Yehong Han¹, Hongyuan Yan³

Affiliations

¹ Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, College of Pharmaceutical Sciences, Hebei University, Baoding 071002, China.
² Key Laboratory of Public Health Safety of Hebei Province, College of Public Health, Hebei University, Baoding, 071002, China.
³ Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, College of Pharmaceutical Sciences, Hebei University, Baoding 071002, China; Key Laboratory of Public Health Safety of Hebei Province, College of Public Health, Hebei University, Baoding, 071002, China. Electronic address: yanhy@hbu.edu.cn.

PMID: 36652815
DOI: 10.1016/j.chroma.2023.463787

Abstract

Alectinib and its metabolite, M4, have demonstrated a satisfactory clinical therapeutic effect in the treatment of anaplastic lymphoma kinase-positive advanced non-small-cell lung cancer. Due to individual differences among patients, therapeutic drug monitoring (TDM) is critical for guaranteeing appropriate clinical drug use. To realize TDM for alectinib and its metabolite, M4, a honeycomb phenol-formaldehyde resin (PFR) with excellent hydrophilic properties, abundant adsorption force, and a stable porous structure was synthesized by modifying the porogens F127 and P123. The prepared PFR was employed as an adsorbent in a simple and efficient spin-column solid-phase extraction (SPE) process. A rapid method for detecting alectinib and its metabolite M4 in urine was thereby established. The established method showed a linear range of 0.0200 μg mL^-1-5.00 μg mL^-1 and the recovery range of 98.8-103% for spiked urine samples, with relative standard deviations of ≤ 4.87% (n = 3). Our results proved the practicability of the proposed honeycomb-PFR spin-column SPE method in TDM for alectinib and its metabolite, M4.

Keywords: Alectinib; Honeycomb resin; Metabolite M4; Spin-column solid-phase extraction; Therapeutic drug monitoring.

MeSH terms

Carcinoma, Non-Small-Cell Lung* / drug therapy
Humans
Lung Neoplasms* / drug therapy
Piperidines
Solid Phase Extraction

Substances

alectinib
Piperidines
phenol-formaldehyde resin