Identification and quantification of DNA N6-methyladenine modification in mammals: A challenge to modern analytical technologies

Cong Lyu; Hui-Dong Wang; Weiyi Lai; Hailin Wang

doi:10.1016/j.cbpa.2022.102259

Identification and quantification of DNA N⁶-methyladenine modification in mammals: A challenge to modern analytical technologies

Curr Opin Chem Biol. 2023 Apr:73:102259. doi: 10.1016/j.cbpa.2022.102259. Epub 2023 Jan 16.

Authors

Cong Lyu¹, Hui-Dong Wang¹, Weiyi Lai¹, Hailin Wang²

Affiliations

¹ The State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China.
² The State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China; School of Environment, Hangzhou Institute for Advanced Study, UCAS, Hangzhou, 310024, China; University of Chinese Academy of Sciences, Beijing, China. Electronic address: hlwang@rcees.ac.cn.

PMID: 36652775
DOI: 10.1016/j.cbpa.2022.102259

Abstract

DNA N⁶-methyladenine modification (6mA) is a predominant epigenetic mark in prokaryotes but rarely present in multicellular metazoa. The analytical technologies have been developed for sensitive detection of 6mA, including ultra-high performance liquid chromatography coupled with mass spectrometry (UHPLC-MS/MS) and single molecule real-time sequencing (SMRTseq). However, it remains challenging to detect 6mA at global level and/or in the context of sequence in multicellular metazoa (including mammals). This mini-review brings insights into current dilemma and potential solutions for the identification and quantifications of 6mA in mammals.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

Animals
DNA Methylation
DNA* / chemistry
Mammals / genetics
Tandem Mass Spectrometry*

Substances

6-methyladenine
DNA