Esterase D interacts with metallothionein 2A and inhibits the migration of A549 lung cancer cells in vitro

J Cell Biochem. 2023 Mar;124(3):373-381. doi: 10.1002/jcb.30371. Epub 2023 Jan 17.

Abstract

Esterase D (ESD) is a nonspecific esterase widely distributed in various organisms. ESD plays an important role in regulating cholesterol efflux, inhibiting viral replication and lung cancer growth. MT2A (metallothionein 2A) is the most important isoform of metallothionein (MTs) in human and high expression of MT2A in tumors represents poor prognosis and metastatic behavior. However, there are no reports about the molecular mechanism of ESD in the regulation of tumor metastasis. In this study, we found for the first time that activation ESD promoted its interaction with MT2A and decreased the protein level of MT2A, which resulting in the concentration of free zinc ions up-regulated, and inhibited the migration of A549 lung cancer cells in vitro.

Keywords: A549 lung cancer cells; Esterase D; metallothionein 2A; migration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • A549 Cells
  • Carboxylesterase* / genetics
  • Carboxylesterase* / metabolism
  • Cell Line, Tumor
  • Cell Movement / genetics
  • Cell Movement / physiology
  • Humans
  • Lung Neoplasms* / genetics
  • Lung Neoplasms* / metabolism
  • Metallothionein* / genetics
  • Metallothionein* / metabolism

Substances

  • Metallothionein
  • ESD protein, human
  • Carboxylesterase
  • MT2A protein, human