Genome-wide characterization, chromosome localization, and expression profile analysis of poplar non-specific lipid transfer proteins

Hui Wei; Guoyuan Liu; Jin Qin; Yanyan Zhang; Jinxin Chen; Xingyue Zhang; Chunmei Yu; Yanhong Chen; Bolin Lian; Fei Zhong; Ali Movahedi; Jian Zhang

doi:10.1016/j.ijbiomac.2023.123226

Genome-wide characterization, chromosome localization, and expression profile analysis of poplar non-specific lipid transfer proteins

Int J Biol Macromol. 2023 Mar 15:231:123226. doi: 10.1016/j.ijbiomac.2023.123226. Epub 2023 Jan 11.

Authors

Hui Wei¹, Guoyuan Liu², Jin Qin², Yanyan Zhang³, Jinxin Chen⁴, Xingyue Zhang⁵, Chunmei Yu⁶, Yanhong Chen⁷, Bolin Lian⁸, Fei Zhong⁹, Ali Movahedi¹⁰, Jian Zhang¹¹

Affiliations

¹ Key Laboratory of Landscape Plant Genetics and Breeding, School of Life Sciences, Nantong University, Nantong, China. Electronic address: weihui2021@ntu.edu.cn.
² Key Laboratory of Landscape Plant Genetics and Breeding, School of Life Sciences, Nantong University, Nantong, China.
³ College of Biology and the Environment, Nanjing Forestry University, Nanjing, China. Electronic address: zyy190103128@njfu.edu.cn.
⁴ Key Laboratory of Landscape Plant Genetics and Breeding, School of Life Sciences, Nantong University, Nantong, China. Electronic address: 2109110019@stmail.ntu.edu.cn.
⁵ Key Laboratory of Landscape Plant Genetics and Breeding, School of Life Sciences, Nantong University, Nantong, China. Electronic address: 2109110016@stmail.ntu.edu.cn.
⁶ Key Laboratory of Landscape Plant Genetics and Breeding, School of Life Sciences, Nantong University, Nantong, China. Electronic address: ychmei@ntu.edu.cn.
⁷ Key Laboratory of Landscape Plant Genetics and Breeding, School of Life Sciences, Nantong University, Nantong, China. Electronic address: chenyh@ntu.edu.cn.
⁸ Key Laboratory of Landscape Plant Genetics and Breeding, School of Life Sciences, Nantong University, Nantong, China. Electronic address: lianbolin@ntu.edu.cn.
⁹ Key Laboratory of Landscape Plant Genetics and Breeding, School of Life Sciences, Nantong University, Nantong, China. Electronic address: fzhong@ntu.edu.cn.
¹⁰ College of Biology and the Environment, Nanjing Forestry University, Nanjing, China. Electronic address: ali_movahedi@njfu.edu.cn.
¹¹ Key Laboratory of Landscape Plant Genetics and Breeding, School of Life Sciences, Nantong University, Nantong, China. Electronic address: yjnkyy@ntu.edu.cn.

PMID: 36641014
DOI: 10.1016/j.ijbiomac.2023.123226

Abstract

Plant non-specific lipid transfer proteins (nsLTPs) are small and have a broad biological function involved in reproductive development and abiotic stress resistance. Although a small part of plant nsLTPs have been identified, these proteins have not been characterized in poplar at the genomic level. A genome-wide characterization and expression identification of poplar nsLTP members were performed in this study. A total of 42 poplar nsLTP genes were identified from the poplar genome. A comprehensive analysis of poplar nsLTPs was conducted by a phylogenetic tree, duplication events, gene structures, and conserved motifs. The cis-elements of poplar nsLTPs were predicted to respond to light, hormone, and abiotic stress. Many transcription factors (TFs) were identified to interact with poplar nsLTP cis-elements. The tested poplar nsLTPs were expressed in leaves, stems, and roots, but their expression levels differed among tested tissues. Most poplar nsLTP expression levels were changed by abiotic stress, implying that poplar nsLTP may be involved in abiotic stress resistance. Network analysis showed that poplar nsLTPs are putative genes involved in fatty acid (FA) metabolism. This research provides sight into the further study to explain the regulatory mechanism of the poplar nsLTPs.

Keywords: Abiotic stress; Expression identification; Poplar; nsLTPs.

MeSH terms

Chromosomes
Gene Expression Regulation, Plant
Lipids
Phylogeny
Plant Proteins* / chemistry
Plants* / genetics
Stress, Physiological

Substances

Plant Proteins
Lipids