Oleocanthal alleviated lipopolysaccharide-induced acute lung injury in chickens by inhibiting TLR4/NF-κB pathway activation

Poult Sci. 2023 Mar;102(3):102458. doi: 10.1016/j.psj.2022.102458. Epub 2022 Dec 30.

Abstract

This study aimed to investigate the ameliorative effect of oleocanthal (OC) on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in chickens and its possible mechanisms. In total, 20 chickens were randomly divided into 4 groups: control (CON) group, LPS group, LPS + OC group, and OC group. LPS + OC and OC groups were intragastrically administered a 5 mg/kg·d OC dose for 7 d. On d 8, the LPS group and LPS + OC group were intratracheally administered 2 mg/kg LPS for 12 h. It was found that OC ameliorated the pathological morphology and significantly suppressed apoptosis after OC treatment in LPS-induced ALI chicken (P < 0.01). Antioxidant capacity was higher in the LPS + OC group compared with the LPS group (P < 0.01). OC downregulated the related genes and proteins expression of toll-like receptor 4/nuclear factor-κB (TLR4/NF-κB) pathway in LPS group (P < 0.01). In conclusion, OC supplementation can alleviate LPS-induced ALI in chickens by suppressing apoptosis, enhancing lung antioxidant capacities and inhibiting TLR4/NF-κB pathway activation.

Keywords: acute lung injury; chicken; lipopolysaccharide; oleocanthal; toll-like receptor 4/nuclear factor-κB.

Publication types

  • Randomized Controlled Trial, Veterinary

MeSH terms

  • Acute Lung Injury* / chemically induced
  • Acute Lung Injury* / drug therapy
  • Acute Lung Injury* / veterinary
  • Animals
  • Antioxidants / metabolism
  • Chickens / metabolism
  • Lipopolysaccharides / toxicity
  • Lung / metabolism
  • NF-kappa B* / genetics
  • NF-kappa B* / metabolism
  • Signal Transduction
  • Toll-Like Receptor 4 / genetics
  • Toll-Like Receptor 4 / metabolism

Substances

  • NF-kappa B
  • Lipopolysaccharides
  • Toll-Like Receptor 4
  • oleocanthal
  • Antioxidants