Pyropia yezoensis-derived porphyran attenuates acute and chronic colitis by suppressing dendritic cells

Hae-Bin Park; So-Jung Kim; Dhananjay Yadav; Eun-Koung An; Wei Zhang; Hee-Yun Eom; Minseok Kwak; Tatsuya Oda; Peter Chang-Whan Lee; Jun-O Jin

doi:10.1016/j.ijbiomac.2023.123148

Pyropia yezoensis-derived porphyran attenuates acute and chronic colitis by suppressing dendritic cells

Int J Biol Macromol. 2023 Mar 15:231:123148. doi: 10.1016/j.ijbiomac.2023.123148. Epub 2023 Jan 11.

Authors

Hae-Bin Park¹, So-Jung Kim², Dhananjay Yadav³, Eun-Koung An², Wei Zhang⁴, Hee-Yun Eom², Minseok Kwak⁵, Tatsuya Oda⁶, Peter Chang-Whan Lee⁷, Jun-O Jin⁸

Affiliations

¹ Shanghai Public Health Clinical Center, Shanghai Medical College, Fudan University, Shanghai 201508, China; Department of Medical Biotechnology, Yeungnam University, Gyeongsan 38541, South Korea; Department of Microbiology, University of Ulsan College of Medicine, Seoul 05505, South Korea.
² Department of Medical Biotechnology, Yeungnam University, Gyeongsan 38541, South Korea; Department of Microbiology, University of Ulsan College of Medicine, Seoul 05505, South Korea.
³ Department of Medical Biotechnology, Yeungnam University, Gyeongsan 38541, South Korea.
⁴ Shanghai Public Health Clinical Center, Shanghai Medical College, Fudan University, Shanghai 201508, China.
⁵ Department of Chemistry, Pukyong National University, Busan 48513, South Korea.
⁶ Graduate School of Fisheries Science and Environmental Studies, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan.
⁷ Department of Biomedical Sciences, University of Ulsan College of Medicine, ASAN Medical Center, Seoul 05505, South Korea. Electronic address: pclee@amc.seoul.kr.
⁸ Shanghai Public Health Clinical Center, Shanghai Medical College, Fudan University, Shanghai 201508, China; Department of Microbiology, University of Ulsan College of Medicine, Seoul 05505, South Korea. Electronic address: junojin@amc.seoul.kr.

PMID: 36639074
DOI: 10.1016/j.ijbiomac.2023.123148

Abstract

Porphyran is known to inhibit immune cell function. Previously, porphyran was shown to prevent lipopolysaccharide-induced sepsis in mice. However, studies on the inhibitory effects of porphyran during colitis are currently lacking. In this study, we evaluated the effects of Pyropia yezoensis-derived porphyran on dextran sodium sulfate (DSS)-induced acute and chronic colitis. The oral or intraperitoneal administration of porphyran inhibited the progression of DSS-induced colitis in mice, with the former also preventing immune cell infiltration in the colon. The levels of intracellular interferon-γ and interleukin-17 in T cells decreased when porphyran was administered orally. Porphyran inhibited T cell activation by suppressing dendritic cells (DCs) and macrophages. Porphyran prevented pathogen-associated molecular pattern and damage-associated molecular pattern-dependent DC and macrophage activation. Finally, porphyran attenuated chronic colitis caused via the long-term administration of DSS. These findings indicate that the oral administration of porphyran can inhibit DSS-induced colitis by suppressing DC and macrophage activation.

Keywords: Autoimmune disease; Colitis; Dendritic cells; Helper T cells; Porphyran.

MeSH terms

Animals
Colitis* / chemically induced
Colon
Dendritic Cells
Dextran Sulfate / adverse effects
Disease Models, Animal
Mice
Mice, Inbred C57BL
Rhodophyta*
Sepharose / pharmacology

Substances

porphyran
Sepharose
Dextran Sulfate