Correlation of mineral density and elastic modulus of dog dentin using μ-CT and nanoindentation

Jason W Soukup; Justin Jeffery; Sienna R Drizin; Scott J Hetzel; Donald S Stone; Melih Eriten; Heidi-Lynn Ploeg; Corinne R Henak

doi:10.1016/j.jbiomech.2023.111434

Correlation of mineral density and elastic modulus of dog dentin using μ-CT and nanoindentation

J Biomech. 2023 Jan:147:111434. doi: 10.1016/j.jbiomech.2023.111434. Epub 2023 Jan 7.

Authors

Jason W Soukup¹, Justin Jeffery², Sienna R Drizin³, Scott J Hetzel⁴, Donald S Stone⁵, Melih Eriten⁶, Heidi-Lynn Ploeg⁷, Corinne R Henak⁸

Affiliations

¹ Department of Surgical Sciences, University of Wisconsin-Madison, School of Veterinary Medicine, Madison, WI, USA. Electronic address: jason.soukup@wisc.edu.
² Carbone Cancer Center, University of Wisconsin-Madison, School of Medicine and Public Health, Madison, WI, USA.
³ Department of Surgical Sciences, University of Wisconsin-Madison, School of Veterinary Medicine, Madison, WI, USA.
⁴ Department of Biostatistics and Medical Informatics, University of Wisconsin-Madison, School of Medicine and Public Health, Madison, WI, USA.
⁵ Department of Materials Science and Engineering, University of Wisconsin-Madison, College of Engineering, Madison, WI, USA.
⁶ Department of Mechanical Engineering, University of Wisconsin-Madison, College of Engineering, Madison, WI, USA.
⁷ Department of Mechanical Engineering, University of Wisconsin-Madison, College of Engineering, Madison, WI, USA; Department of Mechanics and Materials Engineering, Queen's University, Kingston, ON, Canada.
⁸ Department of Mechanical Engineering, University of Wisconsin-Madison, College of Engineering, Madison, WI, USA; Department of Orthopedics and Rehabilitation, University of Wisconsin-Madison, School of Medicine and Public Health, Madison, WI, USA.

Abstract

This study sought to 1) investigate the spatial distribution of mineral density of dog dentin using µ-CT and 2) characterize the relationship between the elastic modulus and mineral density of dog dentin using nanoindentation and µ-CT. Maxillary canine teeth of 10 mature dogs were scanned with a µ-CT then sectioned in the transverse and vertical planes and tested using nanoindentation. Spatial distribution of mineral density and elastic modulus was quantified. Results demonstrated significant spatial variation in mineral density and elastic modulus. Mineral density and elastic modulus generally increased from the dentin-pulp interface to the dentino-enamel junction and from the crown base to the crown tip. Significant site dependent correlations between mineral density and elastic modulus were determined (0.021 > R² > 0.408). The results of this study suggest that while mineral density is a mediator of elastic modulus, other mediators such as collagen content may contribute to the mechanical behavior of dog dentin.

Keywords: Dentin; Elastic modulus; Elasticity-density relationship; Microcomputed tomography; Mineral density; Nanoindentation; µ-CT; ρ(QCT).

Publication types

Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Dentin* / diagnostic imaging
Dogs
Elastic Modulus
Hardness
Minerals
Tomography, X-Ray Computed
Tooth*

Substances

Minerals

Abstract

Publication types

MeSH terms

Substances

Grants and funding