Insights into methanogenesis of mesophilic-psychrophilic varied anaerobic digestion of municipal sludge with antibiotic stress

Hui Yun; Bin Liang; Zhangwei He; Minghan Li; Simin Zong; Zhenfei Wang; Bin Ge; Peng Zhang; Xiangkai Li; Aijie Wang

doi:10.1016/j.jenvman.2023.117278

Insights into methanogenesis of mesophilic-psychrophilic varied anaerobic digestion of municipal sludge with antibiotic stress

J Environ Manage. 2023 Apr 1:331:117278. doi: 10.1016/j.jenvman.2023.117278. Epub 2023 Jan 11.

Authors

Hui Yun¹, Bin Liang², Zhangwei He³, Minghan Li⁴, Simin Zong⁵, Zhenfei Wang¹, Bin Ge¹, Peng Zhang⁶, Xiangkai Li⁷, Aijie Wang⁸

Affiliations

¹ Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, 730000, China; Key Laboratory for Resources Utilization Technology of Unconventional Water of Gansu Province, Gansu Academy of Membrane Science and Technology, Lanzhou, 730020, China; Gansu Key Laboratory of Biomonitoring and Bioremediation for Environment Pollution, School of Life Sciences, Lanzhou University, Lanzhou, 730000, China.
² State Key Laboratory of Urban Water Resource and Environment, School of Civil & Environmental Engineering, Harbin Institute of Technology Shenzhen, Shenzhen, 518055, China.
³ Shaanxi Key Laboratory of Environmental Engineering, School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an, 710055, China.
⁴ State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin, 150001, China.
⁵ Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, 730000, China.
⁶ Key Laboratory for Resources Utilization Technology of Unconventional Water of Gansu Province, Gansu Academy of Membrane Science and Technology, Lanzhou, 730020, China.
⁷ Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, 730000, China; Key Laboratory for Resources Utilization Technology of Unconventional Water of Gansu Province, Gansu Academy of Membrane Science and Technology, Lanzhou, 730020, China; Gansu Key Laboratory of Biomonitoring and Bioremediation for Environment Pollution, School of Life Sciences, Lanzhou University, Lanzhou, 730000, China. Electronic address: xkli@lzu.edu.cn.
⁸ State Key Laboratory of Urban Water Resource and Environment, School of Civil & Environmental Engineering, Harbin Institute of Technology Shenzhen, Shenzhen, 518055, China; Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China. Electronic address: waj0578@hit.edu.cn.

PMID: 36634423
DOI: 10.1016/j.jenvman.2023.117278

Abstract

Methane production through anaerobic digestion (AD) of municipal sludge is economic and eco-friendly, which is commonly affected by temperature and pollutants residues. However, little is known about methanogenesis in psychrophilic AD (PAD) with temperature variations that simulating seasonal variations and with antibiotic stress. Here, two groups of AD systems with oxytetracycline (OTC) were operated with temperature maintained at 35 °C and 15 °C or variation to explore the influence to methanogenesis. The acetic acid was noticeably accumulated in OTC groups initially (P < 0.001). Methane production was noticeably inhibited initially in PAD with OTC, but had been stimulated later at 35 °C. The dominant acetoclastic methanogens Methanosaeta gradually decreased to 15.48% and was replaced by methylotrophic Methanomethylovorans (73.43%) in PAD with OTC. Correspondingly, the abundances of functional genes related to methylotrophic methanogenesis were also higher in these groups. Besides, genes involving in methane oxidation had over 50 times higher abundances in PAD with OTC groups in the second phase. Further investigation is essential to understand the main dynamics of methanogenesis in PAD and to clear the related molecular mechanism for future methane production regulation in sludge systems.

Keywords: Mesophilic-psychrophilic anaerobic digestion; Methanogenesis; Oxytetracycline stress; Temperature variation.

MeSH terms

Anaerobiosis
Anti-Bacterial Agents
Bioreactors
Methane
Oxytetracycline*
Sewage* / chemistry

Substances

Sewage
Anti-Bacterial Agents
Oxytetracycline
Methane