Electrophysiological measurements of synaptic connectivity and plasticity in the longitudinal dentate gyrus network from mouse hippocampal slices

Yoon Ji Kwon; Sojeong Pak; Sunggu Yang; Sungchil Yang

doi:10.1016/j.xpro.2022.102030

Electrophysiological measurements of synaptic connectivity and plasticity in the longitudinal dentate gyrus network from mouse hippocampal slices

STAR Protoc. 2023 Mar 17;4(1):102030. doi: 10.1016/j.xpro.2022.102030. Epub 2023 Jan 11.

Authors

Yoon Ji Kwon¹, Sojeong Pak², Sunggu Yang³, Sungchil Yang⁴

Affiliations

¹ Department of Neuroscience, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong SAR, China; Botamedi Brain Health and Medical Care Company Limited, 50 Stanley Street, Central, Hong Kong SAR, China.
² Department of Neuroscience, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong SAR, China.
³ Department of Nano-bioengineering, Incheon National University, Incheon, South Korea. Electronic address: sungchil.yang@cityu.edu.hk.
⁴ Department of Neuroscience, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong SAR, China. Electronic address: sungguyang@inu.ac.kr.

Abstract

Longitudinal synaptic connections between dentate gyrus (DG) granule neurons in the hippocampus have been found to be correlated with increased anxiety. Here, we present a protocol to assess synaptic connectivity and plasticity in the longitudinal DG network. We detail the steps for (1) obtaining acute mouse hippocampal slices that contain longitudinal DG-DG connections, (2) measuring excitatory postsynaptic potentials using whole-cell patch clamp recording combined with two-photon microscopy and glutamate uncaging, and (3) assessing synaptic plasticity using extracellular field recording. For complete details on the use and execution of this protocol, please refer to Pak et al. (2022).¹.

Keywords: Biophysics; Cell Biology; Microscopy; Neuroscience.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Dentate Gyrus*
Glutamic Acid
Hippocampus* / physiology
Mice
Neuronal Plasticity / physiology
Neurons / physiology

Substances

Glutamic Acid