Photo-regulated genetic encoding of dibenzo[ c,g][1,2]diazocine on proteins via configuration switching

Tingting Zheng; Jielin Fu; Qin Xiong; Xin Shen; Baolin Li; Xiaohu Zhao; Zhipeng Yu

doi:10.1039/d2cc06738b

Photo-regulated genetic encoding of dibenzo[ c,g][1,2]diazocine on proteins via configuration switching

Chem Commun (Camb). 2023 Jan 26;59(9):1201-1204. doi: 10.1039/d2cc06738b.

Authors

Tingting Zheng¹, Jielin Fu¹, Qin Xiong¹, Xin Shen¹, Baolin Li¹, Xiaohu Zhao¹, Zhipeng Yu¹

Affiliation

¹ Key Laboratory of Green Chemistry and Technology of Ministry of Education, College of Chemistry, Sichuan University, 29 Wangjiang Road, Chengdu 610064, P. R. China. zhipengy@scu.edu.cn.

PMID: 36629150
DOI: 10.1039/d2cc06738b

Abstract

We report two evolved Methanosarcina mazei pyrrolysine tRNA synthetases to genetically incorporate the isomers of dibenzo[c,g][1,2]diazocine-alanine (DBDAA) into proteins either in the dark or under regulation of 405 nm photo-stimulation. The genetic-encoded DBDAA realizes photo-tuning of enzymatic activity via the host-guest recognition of cucurbit[7]uril.

MeSH terms

Amino Acyl-tRNA Synthetases* / genetics
Archaeal Proteins* / genetics
Archaeal Proteins* / metabolism
Methanosarcina / genetics

Substances

Amino Acyl-tRNA Synthetases
Archaeal Proteins