Reduced acetic acid formation using NaHSO3 as a steering agent by Actinobacillus succinogenes GXAS137

Abstract

The high production of acetic acid (AC) as a by-product leads to difficult separation and purification of succinic acid (SA) and increases production costs in SA fermentation by Actinobacillus succinogenes. NaHSO₃ as a steering agent was used to reduce AC production. Herein, the optimum fermentation conditions were achieved by single-factor and orthogonal tests as follows: glucose 60 g/L; MgCO₃ 60 g/L; NaHSO₃ 0.15% (w/v); and NaHSO₃ addition time, 8 h after inoculation. After optimization, the SA and AC contents were 44.42 and 5.73 g/L. The SA improved by 100.72%, the AC decreased by 21.18% compared with the unfermented. The acetate kinase activity decreased by 14.36% and acetyl-CoA content improved by 97.55% in the group of NaHSO₃ addition compared with control check (CK). The mechanism of NaHSO₃ is formation acetaldehyde-sodium bisulfite compound and reduction the activity of acetate kinase. These findings indicated a new way of using NaHSO₃ as a steering agent to reduce AC generation and may help promote the development of SA industrial production.

Keywords: Acetaldehyde-sodium bisulfite compound; Acetate kinase; Actinobacillus succinogenes; NaHSO(3); Orthogonal test; Succinic acid.