We currently reporting a simple and first label free optical insulin biosensor using a novel cum naturally existing Chromene mimic receptors via one pot greener methodology. Among synthesized 4H- Chromene derivatives, 2-amino-5,7-dihydroxy-4-(4-nitrophenyl)-4H-chromene-3-carbonitrile (Compound 1c) is showed a very good colorimetric response for insulin within the linear range of 20 fM -500 pM along with theoretically measured LOD of 15.38 fM based on the increment in absorbance. Meanwhile 2-amino-5, 7-dihydroxy-4-(pyren-2-yl)-4H-chromene-3-carbonitrile (Compound 1a) is depicted an excellent quenching in fluorescence response for insulin with LOD of 7.07 fM and along with wide working range from 10 fM -600 pM. The developed biosensor is tested for the detection of insulin in human blood serum using direct as well as standard addition method and the recovery results are showed a very good agreement with standard clinical chemiluminescent assay method. Hence, this developed biosensor is more reliable for clinical detection of Insulin and so a good analytical method for Diabetes diagnosis within a short period of time.
Keywords: Blood serum; Chromene; Diabetes; Fluorescence; Insulin; Optical biosensor.
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